AB133036

Protein A ELISA Kit

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Protein A ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Protein A in in samples.

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Sandwich ELISA - Protein A ELISA Kit (AB133036)

sELISA

Supplier Data

Sandwich ELISA - Protein A ELISA Kit (AB133036)

Representative Standard Curve using ab133036.

Key facts

検出方法

Colorimetric

アッセイタイプ

Sandwich (quantitative)

検出感度

= 3.35 pg/mL

検出範囲

15.63 - 1000 pg/mL

アッセイ時間

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Protein A in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of natural and recombinant Protein A in neutralized buffers.

A chicken IgY antibody specific to Protein A has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. A Protein A polyclonal antibody is then added, incubated and washed. An HRP conjugated antibody is then added incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound

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精度

[ { "reproducibilityType": "Inter", "sample": "High", "replicates": 0, "mean": "378.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8" }, { "reproducibilityType": "Inter", "sample": "Low", "replicates": 0, "mean": "103.6 pg/mL", "standardDeviation": null, "coefficientOfVariability": "13.4" }, { "reproducibilityType": "Inter", "sample": "Media", "replicates": 0, "mean": "209.7 pg/mL", "standardDeviation": null, "coefficientOfVariability": "8.1" }, { "reproducibilityType": "Intra", "sample": "High", "replicates": 0, "mean": "727.8 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Low", "replicates": 0, "mean": "127.9 pg/mL", "standardDeviation": null, "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Media", "replicates": 0, "mean": "364.3 pg/mL", "standardDeviation": null, "coefficientOfVariability": "6.4" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab133036-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Unit", "name":"Plate Sealer", "number":"AB133036-CMP02", "productcode":"" }, { "size":"1 x 100 mL", "name":"20X Wash Buffer Concentrate", "number":"AB133036-CMP09", "productcode":"" }, { "size":"1 x 55 mL", "name":"Assay Buffer 13", "number":"AB133036-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protein A Standard", "number":"AB133036-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"Protein A HRP-Streptavidin Conjugate", "number":"AB133036-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"Protein A Antibody", "number":"AB133036-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Chicken anti-Protein A Microplate (12 x 8 wells)", "number":"AB133036-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Stop Solution 2", "number":"AB133036-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Substrate", "number":"AB133036-CMP08", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

See full target information SpA

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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websiteProtocolBooklet

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Protein A ELISA Kit