AB279933

Phospho-SMAD5 (S463/465) ELISA Kit

Name: Phospho-SMAD5 (S463/465) ELISA Kit (ab279933) | Abcam
Brand: Abcam Limited
SKU: AB279933
Price: 161000 JPY
Availability: InStock

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Phospho-SMAD5 (S463/465) ELISA Kit is a ELISA for the measurement of Phospho-SMAD5 (S463/465) in Human in Cell/Tissue Extracts samples.

別名を表示する

MADH5, SMAD5, SMAD family member 5, SMAD 5, hSmad5, JV5-1, Mothers against decapentaplegic homolog 5, MAD homolog 5, Mothers against DPP homolog 5

2 Images

Sandwich ELISA - Phospho-SMAD5 (S463/465) ELISA Kit (AB279933)

sELISA

Supplier Data

Sandwich ELISA - Phospho-SMAD5 (S463/465) ELISA Kit (AB279933)

HeLa cells were treated with BMP2.

Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-SMAD5 (S463/465) ELISA Kit (AB279933)

Jurkat cells treated with/without CPT.

Jurkat cells were untreated or treated with CPT. Cell lysates were analyzed using this phosphoELISA and Western Blot.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate

交差種

Human

Semi-Quantitative

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Phospho-SMAD5 (S463/465) ELISA Kit (ab279933) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SMAD5 (protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SMAD5. An anti-pan SMAD5 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SMAD5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-SMAD5 (S463/465) antibody is used to detect phosphorylated SMAD5. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAD5 (S463/465) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

SMAD5 also known as Mothers Against Decapentaplegic Homolog 5 is a protein involved in the signal transduction regulated by the transforming growth factor-beta (TGF-beta) superfamily of cytokines. This protein has a molecular mass of approximately 52 kDa and is ubiquitously expressed across various tissues. Mechanically SMAD5 functions as an intracellular mediator that translocates from the cytoplasm to the nucleus upon activation to regulate transcription. It participates actively in transmitting signals from surface receptors into the nucleus influencing gene expression.

Biological function summary

SMAD5 plays a central role in the bone morphogenetic protein (BMP) signaling pathway which is critical for embryonic development and tissue homeostasis. It forms a complex with receptor-regulated SMADs (R-SMADs) and co-SMAD (SMAD4) upon phosphorylation by BMP type I receptors. This complex then moves to the nucleus where it regulates expression of target genes. Through this mechanism SMAD5 impacts processes such as bone formation differentiation and cellular differentiation.

Pathways

Several key cellular signaling processes involve SMAD5. Primarily it contributes to the BMP pathway which is integral to skeletal development and repair. SMAD5 interacts with other SMAD proteins like SMAD1 and SMAD8 which all act downstream of the BMP receptors. Additionally SMAD5 participates in crosstalk with the TGF-beta signaling pathway enabling the fine-tuning of cellular responses to a variety of external cues. This connection places SMAD5 within a network of signaling events important for regulating different cell functions and maintaining cellular homeostasis.

SMAD5's function has links to abnormal bone and cartilage development such as in fibrodysplasia ossificans progressiva (FOP) and pulmonary hypertension (PH). These conditions occur due to dysregulation within the BMP signaling pathway. For example mutations in SMAD5 or its regulatory proteins can cause inappropriate signal propagation leading to ossification in FOP or altered vascular remodeling in PH. The interplay between SMAD5 and SMAD4 another key player in these pathways highlights the complex nature of SMAD-related pathologies.

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製品プロトコール

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ターゲットの情報

Transcriptional regulator that plays a role in various cellular processes including embryonic development, cell differentiation, angiogenesis and tissue homeostasis (PubMed : 12064918, PubMed : 16516194). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 9442019). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33510867). Non-phosphorylated SMAD5 has a cytoplasmic role in energy metabolism regulation by promoting mitochondrial respiration and glycolysis in response to cytoplasmic pH changes (PubMed : 28675158). Mechanistically, interacts with hexokinase 1/HK1 and thereby accelerates glycolysis (PubMed : 28675158).

See full target information SMAD5 phospho S463

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websiteProtocolBooklet

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Phospho-SMAD5 (S463/465) ELISA Kit