Phospho-Smad2 (S245/250/255) and Total Smad2 ELISA Kit

製品の詳細

Phospho-Smad2 (S245/250/255) and Total Smad2 ELISA Kit (ab279930) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Smad2 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Smad2 and total Smad2. An anti-pan Smad2 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Smad2 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho Smad2 (S245/250/255) antibody is added to detect phosphorylated Smad2. In the remaining wells, mouse anti-pan-Smad2 antibody is used to detect pan Smad2. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Smad2 (S245/250/255) or pan Smad2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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