AB279842

Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit

Name: Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (ab279842) | Abcam
Brand: Abcam Limited
SKU: AB279842
Price: 176000 JPY
Availability: InStock

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(1 Publication)

Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit is a ELISA for the measurement of Phospho-JNK1 (T183/Y185) and Total JNK1 in Human in Cell/Tissue Extracts samples.

3 Images

Sandwich ELISA - Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (AB279842)

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (AB279842)

Positive Control.

HepG2 cells were treated with IL-1 beta at 37°C for 30 mins.

Cells were solubilzed at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (AB279842)

HepG2 cells untreated/treated with IL-1 beta.

HepG2 cells were untreated or treated with 25 ng/ml IL-1 beta for 30 mins.

sELISA

Supplier Data

Sandwich ELISA - Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (AB279842)

HepG2 cells untreated/treated with IL-1 beta.

HepG2 cells were untreated or treated with 25 ng/ml IL-1 beta for 30 mins.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate

交差種

Human

Semi-Quantitative

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit (ab279842) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated JNK1 (T183/Y185) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-JNK1 (T183/Y185) and total JNK1. An anti-pan JNK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and JNK1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho JNK1 (T183/Y185) antibody is added to detect phosphorylated JNK1. In the remaining wells, biotinylated anti-pan-JNK1antibody is used to detect pan JNK. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of JNK1 (T183/Y185) or pan JNK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.

Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.

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製品プロトコール

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ターゲットの情報

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 11: PubMed34073895

2021

Artemisinin Protects Porcine Mammary Epithelial Cells against Lipopolysaccharide-Induced Inflammatory Injury by Regulating the NF-κB and MAPK Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wenfei Zhang,Liang Xiong,Jiaming Chen,Zhezhe Tian,Jiaxin Liu,Fang Chen,Man Ren,Wutai Guan,Shihai Zhang

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Phospho-JNK1 (T183/Y185) and Total JNK1 ELISA Kit