PEGylated Protein (Polyethylene Glycol) ELISA Kit (ab133065)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.75 ng/ml - 225 ng/ml
- Sample type: Other biological fluids, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
製品の概要
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製品名
PEGylated Protein (Polyethylene Glycol) ELISA Kit -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% 1 16 25ng/ml 3.4% 2 16 50ng/ml 4.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Expect conc1 3 7.25ng/ml 3.1 5.7% Expect conc1 3 113ng/ml 0.4 2.4% Expect conc1 3 27.7ng/ml 1.4 1.3% -
サンプルの種類
Serum, Plasma, Other biological fluids, Tissue -
アッセイタイプ
Competitive -
検出範囲
1.75 ng/ml - 225 ng/ml -
全工程の試験時間
2h 00m -
ステップ
Multiple steps standard assay -
製品の概要
PEGylated protein ELISA kit is a competitive ELISA designed for the accurate quantitative measurement of PEGylated protein in plasma, serum, tissue lysate samples and other biological samples.
The kit detects both free and conjugated forms of PEG. Due to the nature of the antibody used, this assay is more sensitive to higher molecular weight PEG molecules.
A monoclonal antibody specific to PEGylated protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of PEGylated protein conjugated to biotin, followed by a solution of polyclonal antibody to PEGylated protein. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a blue color. A stop solution changes this color from blue to yellow, and the intensity of this yellow coloration is inversely proportional to the amount of PEGylated protein captured in the plate.
This kit does not contain Tween 20.
Tween 20 usage: Tween 20 is a detergent that can interfere with ELISA and potentially other applications (often used in antibody dilution buffers, such as TBST) when using anti-PEG products. This is also the same case for other polyoxyethylene detergents. Therefore, it is recommended to avoid Tween 20 and other polyoxyethylene detergents when performing ELISA using anti-PEG products.
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特記事項
Poly(ethylene glycol) (PEG) is a widely used polymer in drug delivery systems and is often directly conjugated to a drug therapeutic. PEGylation of drug therapeutics is desirable as it has been found to increase the retention time, reduce the immunogenicity and increase stability towards metabolic enzymes. ab133065, the PEGylated protein ELISA Kit, is applicable for drug development and pharmaceutical manufacturing applications including drug formulations, pharmacokinetics analysis, drug comparison, lead candidate identification, lot release criteria and in-process QC studies. At this time, there are no long-term studies revealing the fate of this polymer in the body. The need for toxicology studies that would determine the accumulation of the metabolized PEGylated therapeutics in various tissues has been identified.
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アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Assay Buffer 39 1 x 25ml 20X Wash Buffer 3 Concentrate 2 x 25ml 500X PEGylated Protein Conjugate 1 x 50µl Biotinylated PEGylated Protein 1 x 5ml PEG Microtiter Plate 1 unit PEGylated BSA Control 1 x 50µl PEGylated Protein Conjugate Diluent 1 x 12ml Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
研究分野
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別名
- Polyethylene Glycol
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133065の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
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Typical Standard CurveRepresentative standard curve using ab133065.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab133065 は 2 報の論文で使用されています。
- Golder MR et al. Reduction of liver fibrosis by rationally designed macromolecular telmisartan prodrugs. Nat Biomed Eng 2:822-830 (2018). PubMed: 30918745
- Wufuer Y et al. GPVI-Fc-PEG improves cerebral infarct volume and cerebral thrombosis in mouse model with cerebral thrombosis. Mol Med Rep 16:7561-7568 (2017). PubMed: 28944903