AB221012

p38 MAPK alpha ELISA Kit

SimpleStep
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(2 Publications)

p38 MAPK alpha ELISA Kit is a single-wash 90-min Simplestep used to quantify p38 MAPK alpha with a sensitivity of 300 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

5 Images

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

ELISA

Supplier Data

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

ELISA

Supplier Data

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

Cell line analysis for Total p38 MAPKα from 200 µg/mL preparations of cell extracts.

Data from triplicate measurements (mean +/- SD) are plotted and compared to 1X Cell Extraction Buffer PTR (zero).

ELISA

Supplier Data

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

Example of p38 MAPKα cell lysate standard curve.

Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).

ELISA

Supplier Data

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

Example of p38 MAPKα recombinant protein standard curve.

Raw data values are fitted to a four-parameter, variable slope curve (+/- SD).

ELISA

Supplier Data

ELISA - p38 MAPK alpha ELISA Kit (AB221012)

Induction of p38 MAPKα (pT180/Y182) phosphorylation in HeLa cells in response to anisomycin treatment.

HeLa cells were cultured in 96-well tissue culture plates and treated (15 min) with a dose-range of anisomycin before cell lysis. Data from quadruplicate measurements of p38 MAPKα (pT180/Y182) are plotted and compared against Total p38 MAPKα protein levels.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Homogenate, Cell Lysate

交差種

Mouse, Human

アッセイタイプ

Semi-quantitative

検出感度

= 300 pg/mL

検出範囲

0.781 - 100 ng/mL

アッセイ時間

1h 1m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

p38 MAPK alpha ELISA Kit ab221012 is a rapid single-wash 90-min Other Immunoassay to measure p38 MAPK alpha in cell lysate, tissue homogenate. This SimpleStep sensitivity is 300 pg/mL.

How the assay works

p38 MAPK alpha SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

p38 MAPK alpha ELISA Kit ab221012 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.2" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab221012-1x96Tests", "assetComponentDetails": [ { "size":"1 x 3 mL", "name":"p38 MAPK alpha (Total) Capture Antibody", "number":"AB221012-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Wash Buffer PT", "number":"AB221012-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB221012-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB221012-CMP09", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution", "number":"AB221012-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB221012-CMP01", "productcode":"" }, { "size":"1 x 12 mL", "name":"5X Cell Extraction Buffer PTR", "number":"AB221012-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized p38 MAPK alpha Control Lysate", "number":"AB221012-CMP04", "productcode":"" }, { "size":"1 x 3 mL", "name":"p38 MAPK alpha (Total) Detector Antibody", "number":"AB221012-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB221012-CMP08", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

P38 also known as MAPK14 is a member of the mitogen-activated protein kinase (MAPK) family. It functions by phosphorylating various downstream substrates and plays a role in cellular responses. The molecular weight of p38 is approximately 38 kDa. This protein is widely expressed across many tissues including heart brain and lungs. Its activation by stimuli such as cytokines and stress factors helps regulate inflammation and cell cycle control.

Biological function summary

P38 is involved in several cellular processes such as inflammation cell differentiation and apoptosis. It often functions as part of a MAPK signaling complex where it serves a critical role in transmitting signals from the cell surface to the nucleus. It interacts with upstream kinases for activation and affects cellular responses by phosphorylating transcription factors and other protein kinases. Through experiments using techniques like p38 western blot and alpha ELISA scientists can monitor its activity and understand its role in cellular physiology.

Pathways

P38 signaling is integral to both the MAPK and NF-kB pathways. It helps mediate several cellular responses including inflammation and stress responses. Within these pathways p38 interacts with other proteins such as JNK and ERK which helps regulate adaptive and innate immune responses. These interactions ensure distinct yet overlapping signaling responses necessary for cellular homeostasis.

P38 plays a role in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis p38 contributes to inflammatory processes promoting the production of pro-inflammatory cytokines. In cancer its role varies; while sometimes promoting cancer cell apoptosis it may also aid in tumor survival and proliferation. The protein TNF-alpha often connects indirectly with p38 through inflammatory pathways highlighting its involvement in these diseases.

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製品プロトコール

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ターゲットの情報

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceuticals (Basel, Switzerland) 15: PubMed35215358

2022

Design, Synthesis, and Biological Evaluation of a Novel VEGFR-2 Inhibitor Based on a 1,2,5-Oxadiazole-2-Oxide Scaffold with MAPK Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Mater H Mahnashi,Fardous F El-Senduny,Mohammed Abdulrahman Alshahrani,Mahrous A Abou-Salim

Evidence-based complementary and alternative medicine : eCAM 2021:5518750 PubMed34745289

2021

Unveiling the Mechanism of Principal Drugs of Lianpu Drink on Chronic Gastritis by Network Pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhan Zhou,Xiaohui Xu,Jiangqin Zeng,Zhiyi Liu,Miao Wang,Wenliang Lv

View all publications

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websiteProtocolBooklet

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Abcam product promise

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p38 MAPK alpha ELISA Kit