AB273254

Ovine IL-4 ELISA Kit

Name: Ovine IL-4 ELISA Kit (ab273254) | Abcam
Brand: Abcam Limited
SKU: AB273254
Price: 115000 JPY
Availability: InStock

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Ovine IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Ovine IL-4 in Sheep in Biofluids, Cell Culture Media samples.

別名を表示する

IL-4, IL4, Interleukin-4, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

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Sandwich ELISA - Ovine IL-4 ELISA Kit (AB273254)

sELISA

Supplier Data

Sandwich ELISA - Ovine IL-4 ELISA Kit (AB273254)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Sheep

アッセイタイプ

Sandwich (quantitative)

検出感度

= 85 pg/mL

検出範囲

0.082 - 20 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Ovine IL-4 ELISA Kit (ab273254) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of ovine IL-4 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for ovine IL-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-ovine IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回収率

[ { "sample": "Serum", "range": "68 - 77 %", "average": "= 72.45" }, { "sample": "Plasma", "range": "69 - 81 %", "average": "= 72.35" }, { "sample": "Cell culture media", "range": "99 - 129 %", "average": "= 112.7" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273254-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273254-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB273254-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273254-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273254-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Ovine IL-4 Antibody", "number":"AB273254-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Ovine IL-4 Standard (Lyophilized)", "number":"AB273254-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Ovine IL-4 coated Microplate (12 x 8 wells)", "number":"AB273254-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273254-CMP03", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273254-CMP04", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

See full target information IL4

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websiteProtocolBooklet

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Abcam product promise

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Ovine IL-4 ELISA Kit