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AB285284

Overall Exosome Capture and Quantification ELISA Assay Kit

Overall Exosome Capture and Quantification ELISA Assay Kit

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Overall Exosome Capture and Quantification ELISA Assay Kit is a Sandwich ELISA for the measurement of Overall Exosome Capture and Quantification ELISA Assay Kit in in Biofluids samples.
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Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 Titration of COLO1 (1) and plasma (2) exosomes. Example of standard curves obtained. Exosome standard preparation derived from (1) COLO1 Cell Culture Supernatant and (2) Plasma of healthy donors. This standard curve is for demonstration only. For quantification purposes, a standard curve must be obtained with every assay.

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • sELISA

Supplier Data

Sandwich ELISA - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 titration of plasma healthy donor exosome standards.

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)
  • WB

Supplier Data

Western blot - Overall Exosome Capture and Quantification ELISA Assay Kit (AB285284)

CD9 marker detection by Western Blotting on lyophilized exosomes from human plasma and the recombinant CD9 protein.

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Key facts

サンプルタイプ

Plasma

アッセイタイプ

Sandwich

検出範囲

0.78125 - 50 µg

アッセイプラットフォーム

Microplate (12 x 8 well strips)

製品の詳細

Overall Exosome Capture and Quantification ELISA Assay Kit ab285284 (previously called ExoQuant Overall Exosome Capture and Quantification ELISA Assay Kit (Human Plasma, Colorimetric), K1201) is a patented double sandwich enzyme-linked immunoassay for quantitative and qualitative analysis of exosomes.

It is a successful platform for exosomes quantification and characterization from small amount of human biological fluids (plasma, serum, urine, saliva) or cell media. It may be exploited to identify exosomes released by cancer cells in the plasma and urine of tumor patients in various disease conditions. The kit consists of ELISA plates pre-coated with proprietary pan-exosome antibodies enabling specific capture of exosomes from different biological samples, including cell culture supernatants and human biological fluids. Quantification and characterization of exosomal proteins is subsequently performed using appropriate detection antibodies against exosome associated antigens that can be for either generic or cell/tissue-specific exosomes. The kit contains Kit contains Lyophilized Exosome Standards for assay calibration. Lyophilized Exosome Standards, characterized for protein content and particle number allow the quantification of unknown sample by a standard calibration curve. Transparent and white plates are available depending on the downstream detection approach (colorimetric and luminometric respectively).

精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 13" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C

製品プロトコール

ターゲットの情報

websiteProtocolBooklet
en

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