AB253210

Mouse TRAIL ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse TRAIL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse TRAIL with a sensitivity of 33.79 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD253, Trail, Tnfsf10, Tumor necrosis factor ligand superfamily member 10, TNF-related apoptosis-inducing ligand, Protein TRAIL

3 Images

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB253210)

sELISA

Unknown

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB253210)

Interpolated concentrations of native TRAIL in mouse kidney and thymus homogenate extract samples based on a 1,000 μg/mL extract load.

The concentrations of TRAIL were measured in duplicate and interpolated from the TRAIL standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TRAIL concentration was determined to be 595.83 pg/mL in kidney homogenate extract and 268.83 pg/mL in thymus homogenate extract.

sELISA

Unknown

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB253210)

Example of mouse TRAIL standard curve in 1X Cell Extraction Buffer PTR.

The TRAIL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse TRAIL ELISA Kit (AB253210)

Example of mouse TRAIL standard curve in Sample Diluent 50BP.

The TRAIL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Extracts, Cell culture media, Citrate plasma, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 33.79 pg/mL

検出範囲

78.125 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse TRAIL ELISA Kit (ab253210) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TRAIL protein in cit plasma, edta plasma, serum, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TRAIL with 33.79 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

TRAIL (Tnfsf10) is a type II transmembrane protein that is a cytokine member of the TNFL superfamily. TRAIL activation leads to NFkappaB induction of apoptosis, making it an attractive target as a drug target for cancer. Chemical induction of TRAIL expression in mice was able to induce a partial reduction in the number of cancerous cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.8" } ]

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回収率

[ { "sample": "Serum", "range": "80 - 94 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "80 - 101 %", "average": "= 87" }, { "sample": "Cell culture media", "range": "111 - 119 %", "average": "= 114" }, { "sample": "Tissue Extracts", "range": "105 - 116 %", "average": "= 110" }, { "sample": "Citrate plasma", "range": "84 - 88 %", "average": "= 86" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TRAIL also known as TNF-related apoptosis-inducing ligand plays an important role in inducing apoptosis. This protein has a mass of around 32 kDa and is expressed across various tissues including immune cells. TRAIL interacts with specific receptors on the cell surface to initiate a signaling cascade that results in programmed cell death. It is an attractive therapeutic target because of its selective ability to induce apoptosis in cancer cells while sparing most normal cells.

Biological function summary

TRAIL's function extends beyond inducing cell death. It is an important member of the tumor necrosis factor (TNF) superfamily which regulates immune responses and inflammation. TRAIL can form a homotrimeric complex allowing it to bind effectively to death receptors on target cells specifically DR4 and DR5 triggering a series of downstream signaling events. This interaction is significant in modulating immune surveillance and tumor suppression.

Pathways

TRAIL is integral to both intrinsic and extrinsic apoptosis pathways. Through these pathways TRAIL is closely related to FADD and caspase-8 which are important for apoptosis induction. The extrinsic pathway involves binding to TRAIL death receptors leading to DISC formation while the intrinsic pathway involves mitochondrial events mediated by members of the Bcl-2 protein family.

TRAIL has a significant connection with cancer and autoimmune diseases. TRAIL plays a dual role in cancer where it acts as a tumor suppressor but can also promote tumor progression if cancer cells become resistant to its effects. In cancer TRAIL’s interaction with proteins like FLIP can alter the apoptotic potential of the cells. In autoimmune diseases TRAIL's dysregulation can contribute to tissue damage due to increased immune activity highlighting its complex role in maintaining immune balance.

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製品プロトコール

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ターゲットの情報

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

See full target information Tnfsf10

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:1325-1343 PubMed38389833

2024

Therapeutic effects of a novel electrode for transcranial direct current stimulation in ischemic stroke mice.

Applications

Unspecified application

Species

Unspecified reactive species

Da Hee Jung,Jae Ho Lee,Hong Ju Lee,Jang Woo Park,Young-Jin Jung,Hwa Kyoung Shin,Byung Tae Choi

Pharmaceuticals (Basel, Switzerland) 16: PubMed37895919

2023

Oxaliplatin Enhances the Apoptotic Effect of Mesenchymal Stem Cells, Delivering Soluble TRAIL in Chemoresistant Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana G Quiroz-Reyes,Paulina Delgado-González,José F Islas,Adolfo Soto-Domínguez,Carlos A González-Villarreal,Gerardo R Padilla-Rivas,Elsa N Garza-Treviño

Biomedicines 11: PubMed36831131

2023

Mesenchymal Stem Cells Genetically Modified by Lentivirus-Express Soluble TRAIL and Interleukin-12 Inhibit Growth and Reduced Metastasis-Relate Changes in Lymphoma Mice Model.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana G Quiroz-Reyes,Carlos A Gonzalez-Villarreal,Alberto Y Limon-Flores,Paulina Delgado-Gonzalez,Herminia G Martinez-Rodriguez,Salvador L Said-Fernandez,Adolfo Soto-Dominguez,Ana M Rivas-Estilla,Jose F Islas,Juan F Molina-De la Garza,Elsa N Garza-Treviño

View all publications

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websiteProtocolBooklet

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Mouse TRAIL ELISA Kit