Mouse Thyroid peroxidase/TPO ELISA Kit (ab302763)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 4.76 pg/ml
- Range: 26.563 pg/ml - 1700 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse Thyroid peroxidase/TPO ELISA Kit
Thyroid Peroxidase/TPO キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 8 9.8% -
サンプルの種類
Tissue Extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
4.76 pg/ml -
検出範囲
26.563 pg/ml - 1700 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Tissue Extracts 110 109% - 111% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse Thyroid peroxidase/TPO SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse Thyroid peroxidase/TPO protein in mouse tissue extract. Quantitate Mouse Thyroid peroxidase/TPO with 4.76 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 96 tests 10X Mouse Thyroid peroxidase/TPO Capture Antibody 1 x 600µl 10X Mouse Thyroid peroxidase/TPO Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Mouse Thyroid peroxidase/TPO Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). -
パスウェイ
Hormone biosynthesis; thyroid hormone biosynthesis. -
関連疾患
Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.
Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. -
配列類似性
Belongs to the peroxidase family. XPO subfamily.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
翻訳後修飾
Glycosylated.
Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.
Cleaved in its N-terminal part. -
細胞内局在
Membrane and Cell surface. - Information by UniProt
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別名
- MSA
- PERT_HUMAN
- TDH2A
see all -
参照データベース
- Entrez Gene: 22018 Mouse
- SwissProt: P35419 Mouse
- Unigene: 4991 Mouse
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302763の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Interpolated concentrations of mouse Thyroid Peroxidase/TPO in mouse thyroid extract.
Interpolated concentration of native Thyroid Peroxidase/TPO was measured in duplicate at different sample concentrations. Undiluted samples are 25 µg/mL mouse thyroid extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Example of mouse Thyroid Peroxidase/TPO standard curve in 1X Cell Extraction Buffer PTR.Example of mouse Thyroid Peroxidase/TPO standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Simple Step Elisa ProtocolSimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Benefits of Recombinant Antibodies
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302763 は論文での使用が確認できていません。