Mouse Surfactant protein D / SP-D ELISA Kit

製品の詳細

The Mouse Surfactant protein D / SP-D Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213890) is designed for the quantitative detection of Mouse Surfactant protein D / SP-D in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A polyclonal antibody from sheep specific for Surfactant protein D / SP-D has been precoated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: A20-F374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for Surfactant protein D / SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Surfactant protein D / SP-D amount of sample captured in plate.

Surfactant, pulmonary-associated protein D, also known as SFTPD or Surfactant protein D / SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the Surfactant protein D / SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SPD include roles in innate immunity and surfactant metabolism, Surfactant protein D / SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and Surfactant protein D / SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.