Mouse, Rat Kallikrein 14 ELISA Kit (ab289901)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.58 pg/ml
- Range: 10.938 pg/ml - 700 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
製品の概要
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製品名
Mouse, Rat Kallikrein 14 ELISA Kit
Kallikrein 14 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 13.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 13.8% -
サンプルの種類
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 2.58 pg/ml -
検出範囲
10.938 pg/ml - 700 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 89 84% - 99% Hep Plasma 100 94% - 103% EDTA Plasma 95 93% - 97% Cit plasma 86 81% - 92% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse, Rat
非交差種: Cow, Human, Monkey -
製品の概要
Mouse, Rat Kallikrein 14 ELISA Kit (ab289901) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse and Rat Kallikrein 14 in cell culture, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse and Rat Kallikrein 14 with 2.58 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse, Rat Kallikrein 14 Capture Antibody 1 x 600µl 10X Mouse, Rat Kallikrein 14 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR2 1 x 6ml Mouse, Rat Kallikrein 14 Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. -
組織特異性
Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻訳後修飾
Proteolytic cleavage of the activation peptide produces the active enzyme. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
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別名
- hK14
- Kallikrein 14
- Kallikrein like protein 6
see all -
参照データベース
- Entrez Gene: 317653 Mouse
- Entrez Gene: 308562 Rat
- SwissProt: Q8CGR5 Mouse
- Unigene: 247377 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289901の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of mouse rat Kallikrein 14 standard curve in Sample Diluent NS.
Example of mouse rat Kallikrein 14 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Kallikrein 14 in rat serum, plasma (citrate), and plasma (heparin).Interpolated concentration of native Kallikrein 14 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: rat serum 1:100, rat plasma (citrate) 1:50, and rat plasma (heparin) 1:100. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of mouse Kallikrein 14 in mouse serum, plasma (citrate), plasma (heparin), and plasma (EDTA).Interpolated concentration of native Kallikrein 14 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: mouse serum 50%, mouse plasma (citrate) 25%, mouse plasma (heparin) 25%, and mouse plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Mouse, Rat Kallikrein 14 ELISA Kit (ab289901)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Mouse, Rat KLK14 ELISA Kit (ab289901) -
Sandwich ELISA - Mouse, Rat Kallikrein 14 ELISA Kit (ab289901)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289901 は論文での使用が確認できていません。