AB263880

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)

Recombinant
SimpleStep
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(31 Publications)

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) is a single-wash 90-min Simplestep used to quantify Mouse/Rat ACTH (adrenocorticotropic hormone) with a sensitivity of 6 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 10 citations

別名を表示する

Adrenocorticotropic hormone, Adrenocorticotropin, Alpha melanocyte stimulating hormone, Beta LPH, Beta melanocyte stimulating hormone, Beta-endorphin, Corticotropin lipotropin, Corticotropin-like intermediary peptide, Gamma LPH, Lipotropin beta, Lipotropin gamma, Lipotropin, included, MSH, Melanocyte-stimulating hormone, included, Melanotropin alpha, Melanotropin beta, Melanotropin gamma, Melanotropin, included, Met-enkephalin, NPP, Opiomelanocortin prepropeptide, POC, POMC, Pomc-1, Pomc2, Pro ACTH endorphin, Pro opiomelanocortin, Pro-opiomelanocortin-alpha, Proopiomelanocortin preproprotein, Tetracosactide, alpha-MSH, beta-MSH, gamma-MSH

4 Images

ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

ELISA

Supplier Data

ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Interpolated concentrations of native ACTH in mouse and rat serum and plasma samples.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 50% and plasma (edta) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 123.02 pg/mL in serum, 433.40 pg/mL in plasma (citrate), 240.48 pg/mL in plasma (heparin) and 328.43 pg/mL in plasma (edta).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Interpolated concentrations of native ACTH in rat serum and plasma samples.

The concentrations of ACTH were measured in duplicates, interpolated from the ACTH standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 15%, plasma (heparin) 15% and plasma (edta) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ACTH concentration was determined to be 1178.40 pg/mL in serum, 2974.54 pg/mL in plasma (citrate), 1988.24 pg/mL in plasma (heparin) and 1508.42 pg/mL in plasma (edta).

sELISA

Supplier Data

Sandwich ELISA - Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) (AB263880)

Example of mouse and rat ACTH standard curve in 1X Cell Extraction Buffer PTR.

The ACTH standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

交差種

Mouse, Rat

アッセイタイプ

Sandwich

検出感度

= 6 pg/mL

検出範囲

7.81 - 500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 is a rapid single-wash 90-min ELISA kit to measure Mouse/Rat ACTH in serum and plasma. This SimpleStep ELISA® sensitivity is 6 pg/mL.

How the assay works

Mouse/Rat ACTH SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes native mouse and rat ACTH in serum and plasma samples only. As ACTH is a product of POMC, this kit is expected to cross-react in any samples that contain the unprocessed precursor. Cell culture supernatant, cell and tissue extract samples have not been tested with this kit.

Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone) ab263880 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse/Rat ACTH ELISA kit ab263880

Mouse/Rat ACTH ELISA kit ab263880 has been used to measure the hypothalamus–pituitary–adrenal axis activation⁽¹⁾⁽²⁾

References:

⁽¹⁾Hoyoung Kim et al. 2023, PMID: 37653040,
⁽²⁾Weiyi Chen et al. 2023, PMID: 37075752

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse/Rat ACTH (adrenocorticotropic hormone) Antibody Pair - BSA and Azide free ab267700

Related and recommended products

Mouse/Rat ELISA kit ab263880 is commonly used to measure the activity of the hypothalamic-pituitary-adrenal axis.

Other ELISA kits to measure related hormones include:

- Cortisol ELISA kit ab108665
- Human Growth Hormone ELISA kit ab190811

ACTH, adrenocorticotropic hormone, is a peptide hormone cleavage product of the POMC protein produced by the pituitary gland. ACTH plays an important role in stress and circadian rhythm signaling. ACTH's primary action is on the adrenal cortex, where its binding induces the secretion of glucocorticoid steroid hormones. The peptide sequence for this hormone is highly evolutionarily conserved amongst mammals. Specifically, for mouse and rat, this sequence shares 100% identity.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.2" } ]

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回収率

[ { "sample": "Serum", "range": "82 - 92 %", "average": "= 87" }, { "sample": "EDTA Plasma", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "113 - 120 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "89 - 107 %", "average": "= 101" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

See full target information ACTH

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文献 (31)

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Mouse/Rat ACTH ELISA Kit (adrenocorticotropic hormone)