AB269553

Mouse RANKL ELISA Kit (TRANCE/TNFSF11)

Recombinant
SimpleStep
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Mouse RANKL ELISA Kit (TRANCE/TNFSF11) is a single-wash 90-min Simplestep used to quantify Mouse RANKL (TRANCE/TNFSF11) with a sensitivity of 1.85 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE

4 Images

Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (AB269553)

sELISA

Supplier Data

Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (AB269553)

Interpolated concentrations of native RANKL in mouse serum and plasma samples.

The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma-citate neat, and plasma-EDTA neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 22.7 pg/mL in serum, and 18.3 pg/mL in plasma-citrate, and 22.3 pg/mL in plasma-EDTA.

sELISA

Supplier Data

Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (AB269553)

Interpolated concentrations of native RANKL in mouse cell culture supernatant samples.

The concentrations of RANKL were measured in duplicates, interpolated from the RANKL standard curves and corrected for sample dilution. Undiluted samples are as follows : neat stimulated splenocytes supernatant and neat unstimulated splenocytes supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANKL concentration was determined to be 28.5 pg/mL in stimulated splenocytes and 14.1 pg/mL in unstimulated splenocytes.

sELISA

Supplier Data

Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (AB269553)

Mouse RANKL standard curve comparison.

Standard Curve comparison between Mouse RANKL SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (AB269553)

Example of mouse RANKL standard curve in Sample Diluent NS.

The RANKL standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 1.85 pg/mL

検出範囲

6.25 - 400 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse RANKL ELISA Kit (TRANCE/TNFSF11) (ab269553) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TRANCE/TNFSF11) protein in cell culture supernatant, edta plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RANKL (TRANCE/TNFSF11) with 1.85 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Receptor activator of nuclear factor kappa-β ligand (RANKL) is a type II membrane protein and belongs to the tumor necrosis factor superfamily. RANKL is known to interact with TNFRS11B/OPG and TNFRSF11A/RANK which controls fundamental aspects of bone physiology and affects the immune system. Some of the specific functions of RANKL include essential regulator of bone remodeling, lymph node formation, and bone metastasis in cancer. ab269553 kit is developed to recognize extracellular soluble RANKL.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.24" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.94" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "102 - 104 %", "average": "= 103" }, { "sample": "Serum", "range": "105 - 107 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "97 - 101 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "96 - 100 %", "average": "= 97" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor (PubMed : 22437732). Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy (By similarity). Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts (PubMed : 18586671, PubMed : 24039232, PubMed : 27336669). During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation (PubMed : 23395171, PubMed : 26644563).

See full target information Tnfsf11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:4820 PubMed35973996

2022

A TrkB agonist prodrug prevents bone loss via inhibiting asparagine endopeptidase and increasing osteoprotegerin.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xiong,Jianming Liao,Xia Liu,Zhaohui Zhang,Jonathan Adams,Roberto Pacifici,Keqiang Ye

View all publications

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websiteProtocolBooklet

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Mouse RANKL ELISA Kit (TRANCE/TNFSF11)