AB214572

Mouse Prolactin/PRL ELISA Kit

Recombinant
SimpleStep
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(3 Publications)

Mouse Prolactin/PRL ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Prolactin/PRL with a sensitivity of 139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor

5 Images

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Comparison of native Prolactin concentrations in pregnant and normal mouse serum.

The concentrations of Prolactin were measured in three 2-fold serial dilutions starting at 25% serum samples in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng of Prolactin per mL of neat serum (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 6.5 ng/mL in pregnant mouse serum and 2.6 ng/mL in normal mouse serum.

sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Interpolated concentrations of spike Prolactin in normal mouse serum samples.

The concentrations of Prolactin were measured in duplicates, interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows : normal mouse serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Example of mouse Prolactin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Interpolated concentrations of native Prolactin in mouse pituitary cell extract based on a 250 ng/mL extract load.

The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prolactin concentration was determined to be 20 ng/mL in mouse pituitary cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Prolactin/PRL ELISA Kit (AB214572)

Example of mouse Prolactin standard curve in1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Serum

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 139 pg/mL

検出範囲

0.562 - 36 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Prolactin/PRL ELISA Kit (ab214572) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Prolactin/PRL protein in cell culture extracts, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Prolactin/PRL with 139 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Prolactin/PRL is a secreted neuroendocrine pituitary hormone. Prolactin acts primarily on the mammary gland by promoting lactation, but has also additional pleiotropic effects both in males and females. Mature prolactin is a 199 amino acid long secreted monomeric protein. Prolactin can be also found in less biologically active form, macroprolactin, in a complex with IgG.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" } ]

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回収率

[ { "sample": "Serum", "range": "69 - 80 %", "average": "= 75" }, { "sample": "Tissue Extracts", "range": "98 - 109 %", "average": "= 102" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab214572-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB214572-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB214572-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB214572-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Prolactin Lyophilized Recombinant Protein", "number":"AB214572-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Prolactin Capture Antibody", "number":"AB214572-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB214572-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Prolactin Detector Antibody", "number":"AB214572-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB214572-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB214572-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB214572-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB214572-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB214572-CMP08", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin (PRL) also known as mammotropin is a peptide hormone with a molecular mass of approximately 23 kDa. PRL is primarily expressed in the anterior pituitary gland but can also be found in other tissues such as the mammary glands brain and immune system. PRL plays an important role in lactation stimulating milk production in mammals. The PRL protein functions by binding to the prolactin receptor a member of the cytokine receptor family initiating signal transduction pathways.

Biological function summary

PRL influences a variety of physiological processes including reproduction metabolism and osmoregulation. It acts as a single entity rather than part of a larger protein complex affecting development and growth through its regulatory activities. In addition to lactation PRL also plays roles in the immune system cell proliferation and the production of surfactant in the lungs.

Pathways

Prolactin is deeply involved in the JAK-STAT signaling pathway and the MAPK/ERK pathway which are pivotal for transmitting signals from the prolactin receptor to the nucleus. Through these pathways PRL interacts with proteins like Janus kinase (JAK) and Signal Transducer and Activator of Transcription (STAT) proteins facilitating its wide range of biological effects. This interplay helps regulate gene expression critical for various physiological conditions.

PRL is linked to conditions such as hyperprolactinemia and prolactinoma. Hyperprolactinemia characterized by elevated levels of prolactin in the blood can lead to symptoms such as galactorrhea and infertility. In the context of prolactinoma an adenoma of the pituitary gland PRL's overproduction is common. These disorders often relate to proteins like dopamine which typically acts to inhibit PRL secretion from the pituitary gland and any disruption in this regulation can result in the aforementioned conditions.

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製品プロトコール

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ターゲットの情報

See full target information Prolactin/PRL

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Basic and clinical andrology 35:9 PubMed40055615

2025

Pelvic vasectomy and its protective effects on rat testis function.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Yang,Yujun Chen,Xiaofeng Cheng,Jingxin Wu,Ruohui Huang,Biao Qian,Gongxian Wang

Journal of neuroinflammation 21:302 PubMed39563331

2024

Microglia-derived ADAM9 promote GHRH neurons pyroptosis by Mad2L2-JNK-caspase-1 pathway in subarachnoid hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Mao,Yun Bao,Fan Liu,Qiyun Ye,Junxiang Peng,Jing Nie,Lijun Huang,Yonghong Liao,Yiheng Xing,Dongyang Wu,Ke Wang,Wenfeng Feng,Songtao Qi,Jun Pan,Binghui Qiu

Cell death & disease 12:879 PubMed34564702

2021

Satb2 regulates the development of dopaminergic neurons in the arcuate nucleus by Dlx1.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhang,Lei Zhang,Ying Huang,Pengcheng Ma,Bingyu Mao,Yu-Qiang Ding,Ning-Ning Song

View all publications

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Mouse Prolactin/PRL ELISA Kit