AB282297

Mouse Prealbumin ELISA Kit

Recombinant
SimpleStep
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Mouse Prealbumin ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Mouse Prealbumin with a sensitivity of 62.48 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab325450

別名を表示する

Transthyretin, Prealbumin, Ttr

6 Images

ELISA - Mouse Prealbumin ELISA Kit (AB282297)

ELISA

Supplier Data

ELISA - Mouse Prealbumin ELISA Kit (AB282297)

sELISA

Supplier Data

Sandwich ELISA - Mouse Prealbumin ELISA Kit (AB282297)

Interpolated concentrations of native Prealbumin in serum, plasma (citrate), plasma (heparin) and plasma (EDTA).

The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 25,000, plasma (citrate) 1 : 25,000, plasma (heparin) 1 : 25,000, and plasma (EDTA) 1 : 25,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 301.90 μg/mL in serum, 168.93 μg/mL in plasma (citrate), 309.51 μg/mL in plasma (heparin), and 264.54 μg/mL in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse Prealbumin ELISA Kit (AB282297)

Interpolated concentrations of native Prealbumin in mouse liver tissue supernatant samples.

The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 1 : 50. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 654.57 ng/mL in liver supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Prealbumin ELISA Kit (AB282297)

Example of mouse Prealbumin standard curve in Sample Diluent NS.

The Prealbumin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit (AB282297)

Example of mouse Prealbumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Mouse Prealbumin ELISA Kit (AB282297)

Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 25,000 mouse serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 62.48 pg/mL

検出範囲

234.38 - 15000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Prealbumin ELISA Kit ab282297 is a rapid single-wash 90-min ELISA kit to measure Mouse Prealbumin in Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.

How the assay works

Mouse Prealbumin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit is designed for the quantification of mouse Prealbumin. The standard protein in this kit is full length mouse Prealbumin. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), and tissue culture supernatant sample types. Cell extract, tissue extract, urine, saliva, CSF, and milk samples have not been tested with this kit.

Mouse Prealbumin ELISA Kit ab282297 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

Save your precious samples

Our 384-well Mouse Prealbumin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse Prealbumin Antibody Pair - BSA and Azide free ab253737
- Anti-Prealbumin antibody [EPR20971-130] - BSA and Azide free (Capture) ab283848
- Anti-Prealbumin antibody [EPR20971-140] - BSA and Azide free (Detector) ab283849

Related and recommended products

Mouse Prealbumin ELISA Kit ab282297 is commonly used to study nutrition and endocrine disorders.

To measure prealbumin in human samples:

- Human Prealbumin ELISA kit ab231920

Prealbumin (Transthyretin, or TTR) is a transport protein for thyroxine and retinol-binding protein bound to retinol. Prealbumin forms a dimer of dimers, and misfolding of it is associated with various amyloid diseases. Prealbumin levels are an indication of protein-calorie nutrition and has been used to monitor the effectiveness of parenteral nutrition.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "100 - 110 %", "average": "= 106" }, { "sample": "Serum", "range": "109 - 118 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "93 - 99 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "91 - 96 %", "average": "= 93" }, { "sample": "Citrate plasma", "range": "102 - 113 %", "average": "= 109" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.

Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.

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製品プロトコール

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ターゲットの情報

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

See full target information Ttr

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Physical science 5: PubMed39421604

2024

Enhancing RNA inhibitory activity using clamp-G-modified nucleobases.

Applications

Unspecified application

Species

Unspecified reactive species

Sai Pallavi Pradeep,Vikas Kumar,Shipra Malik,Frank J Slack,Anisha Gupta,Raman Bahal

View all publications

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Mouse Prealbumin ELISA Kit