AB323695

Mouse PD-L1 ELISA Kit- Extracellular

Recombinant
SimpleStep
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Mouse PD-L1 ELISA Kit- Extracellular is a single-wash 90-min Simplestep used to quantify Mouse PD-L1 with a sensitivity of 6.267 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

CD274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, Cd274, Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1

3 Images

Sandwich ELISA - Mouse PD-L1 ELISA Kit- Extracellular (AB323695)

sELISA

Supplier Data

Sandwich ELISA - Mouse PD-L1 ELISA Kit- Extracellular (AB323695)

Interpolated concentration of native PD-L1 was measured in duplicate at different sample concentrations. Undiluted samples are 100% RAW264.7 cell culture supernatant (treated with 1 µg/ml LPS for 48 hrs) and 100% untreated RAW264.7 cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT. No signal was detected in untreated RAW264.7 cell culture supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse PD-L1 ELISA Kit- Extracellular (AB323695)

Example of mouse PD-L1 standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse PD-L1 ELISA Kit- Extracellular (AB323695)

Example of mouse PD-L1 standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Cell culture supernatant

交差種

Mouse

アッセイタイプ

Sandwich (quantitative)

検出感度

= 6.267 pg/mL

検出範囲

21.875 - 1400 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse PD-L1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PD-L1 protein in Serum, Citrate Plasma, EDTA Plasma, Cell culture supernatant. Quantitate Mouse PD-L1 with 6.267 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
- Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]

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回収率

[ { "sample": "Serum", "range": "90 - 93 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "81 - 88 %", "average": "= 83" }, { "sample": "EDTA Plasma", "range": "88 - 92 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "87 - 105 %", "average": "= 96" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Plays a critical role in induction and maintenance of immune tolerance to self (PubMed : 11238124). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed : 11238124). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed : 11015443, PubMed : 12719480).. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed : 12218188, PubMed : 27281199). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed : 12218188). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed : 12218188).

See full target information Cd274

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websiteProtocolBooklet

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