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AB327296

Mouse MMP-2 ELISA Kit, Chemiluminescent

Mouse MMP-2 ELISA Kit, Chemiluminescent

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Mouse MMP-2 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse MMP-2 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 45.27 - 33000 pg/ml with a sensitivity of 9.71 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

別名を表示する

72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, Mmp2

3 Images
Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Unknown

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Interpolated concentrations of native MMP-2 in mouse serum and plasma samples.

The concentrations of MMP-2 were measured in duplicates interpolated from the MMP-2 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 0.3% plasma (EDTA) 0.3% and plasma (heparin) 0.3%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean MMP-2 concentration was determined to be 824.05 ng/mL in serum 737.92 ng/mL in plasma (EDTA) 884.77 ng/mL in plasma (citrate).

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Lab

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Standard curve comparison between the Chemiluminescent Mouse MMP-2 SimpleStep ELISA and colorimetric Mouse MMP-2 SimpleStep ELISA (ab254516) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)
  • sELISA

Lab

Sandwich ELISA - Mouse MMP-2 ELISA Kit, Chemiluminescent (AB327296)

Dynamic range comparison between the Chemiluminescent Mouse MMP-2 SimpleStep ELISA, colorimetric Mouse MMP-2 SimpleStep ELISA (ab254516), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

検出方法

Luminescent

サンプルタイプ

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma

交差種

Human, Mouse, Rat, Monkey, Cow

アッセイタイプ

Sandwich

Quantitative
検出感度

= 9.71 pg/mL

検出範囲

45.27 - 33000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Mouse MMP-2 ELISA Kit, Chemiluminescent (ab327296) is a rapid single-wash sandwich 55-min ELISA to measure Mouse MMP-2 in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma with a sensitivity of 9.71 pg/mL.

How the assay works

Mouse MMP-2 ELISA Kit, Chemiluminescent (ab327296) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MMP-2 (ab327296) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-MMP2 antibody [EPR17003-25] - BSA and Azide free (Capture) ab259743
- Anti-MMP2 antibody [EPR22283-37] - BSA and Azide free (Detector) ab259744
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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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回収率

[ { "sample": "Cell culture supernatant", "range": "87 - 108 %", "average": "= 95" }, { "sample": "Serum", "range": "83 - 106 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "80 - 118 %", "average": "= 95" }, { "sample": "Heparin Plasma", "range": "85 - 113 %", "average": "= 95" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues (By similarity).. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).. Isoform 2. Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
See full target information Mmp2
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websiteProtocolBooklet
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