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AB327291

Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent

Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent

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Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse MIP2 (CXCL2) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.46 - 1000 pg/ml with a sensitivity of 0.12 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

別名を表示する

Mip-2, Mip2, Scyb2, Cxcl2, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2

6 Images
Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Mouse MIP2 expression is shown for serum and plasma samples.

Native MIP2 was measured in duplicate in 100% serum and 100% heparin plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 50BP. Native MIP2 was measured in duplicate in 100% Citrate plasma and 50% EDTA plasma and concentrations interpolated from a standard curve diluted in Sample Diluent 25BP. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Linearity of dilution of spiked recombinant MIP2 in plasma.

Recombinant mouse MIP2 was spiked into 100% citrate plasma and 50% EDTA plasma and diluted in a 2-fold dilution series in Sample Diluent 25BP. Recombinant mouse MIP2 was spiked into 100% heparin plasma and diluted in a 2-fold dilution series in Sample Diluent 50BP. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Mouse MIP2 expression is shown for cultured media from two mouse cell lines.

J774A.1 cells were cultured in HGDMEM with 10% fetal calf serum and 100 µg/mL of Kanamycin. During the exponential growth phase J774A.1 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. RAW 264.7 cells were cultured in HGDMEM with 10% fetal calf serum 2 mM L-glutamine and 100 µg/mL Kanamycin. During the exponential growth phase RAW264.7 cells were starved for 24 hours and treated in the presence and absence of 5 µg/mL of LPS (Batch #1) or 1% PHA (Batch #2).The concentrations of mouse MIP2 were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Linearity of dilution of MIP2 in serum and cell culture media.

Recombinant mouse MIP2 was spiked into 100% serum and diluted in a 2-fold dilution series in Sample Diluent 50BP. Recombinant mouse MIP2 was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse MIP2 were measured in duplicate and interpolated from the mouse MIP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Lab

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Standard curve comparison between the Chemiluminescent Mouse MIP2 SimpleStep ELISA and colorimetric Mouse MIP2 SimpleStep ELISA (ab204517) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)
  • sELISA

Lab

Sandwich ELISA - Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (AB327291)

Dynamic range comparison between the Chemiluminescent Mouse MIP2 SimpleStep ELISA, colorimetric Mouse MIP2 SimpleStep ELISA (ab204517), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

検出方法

Luminescent

サンプルタイプ

Serum, Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative
検出感度

= 0.12 pg/mL

検出範囲

0.46 - 1000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (ab327291) is a rapid single-wash sandwich 55-min ELISA to measure Mouse MIP2 (CXCL2) in Serum, Cell culture media, Citrate plasma, EDTA Plasma, Heparin Plasma with a sensitivity of 0.12 pg/mL.

How the assay works

Mouse MIP2 (CXCL2) ELISA Kit, Chemiluminescent (ab327291) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse MIP2 (CXCL2) (ab327291) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CXCL2 antibody [EPR17014-91] - BSA and Aside free (Capture) ab242458
- Anti-CXCL2 antibody [EPR17014-241] - BSA and Aside free (Detector) ab242709
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精度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.64" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.87" } ]
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回収率

[ { "sample": "Serum", "range": "103 - 113 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "98 - 107 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "87 - 97 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "113 - 116 %", "average": "= 115" }, { "sample": "Heparin Plasma", "range": "101 - 109 %", "average": "= 104" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.
See full target information Cxcl2
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websiteProtocolBooklet
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Abcam product promise

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