AB253229

Mouse MAP2 ELISA Kit

Recombinant
SimpleStep
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Mouse MAP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse MAP2 with a sensitivity of 43.31 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Mtap2, Map2, Microtubule-associated protein 2, MAP-2

4 Images

Sandwich ELISA - Mouse MAP2 ELISA Kit (AB253229)

sELISA

Unknown

Sandwich ELISA - Mouse MAP2 ELISA Kit (AB253229)

Interpolated concentrations of native MAP2 in mouse brain tissue based on a 15 μg/mL extract load.

The concentrations of MAP2 were measured in duplicate and interpolated from the MAP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAP2 concentration was determined to be 3632.84 pg/mL in mouse brain tissue extract.

sELISA

Unknown

Sandwich ELISA - Mouse MAP2 ELISA Kit (AB253229)

Example of mouse MAP2 standard curve in Sample Diluent NS.

The MAP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse MAP2 ELISA Kit (AB253229)

Representative image of Mouse MAP2 ELISA Kit (ab253229)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Mouse MAP2 Capture Antibody

- 10X Mouse MAP2 Detector Antibody

- Mouse MAP2 Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Sample diluent 5BR

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Unknown

Sandwich ELISA - Mouse MAP2 ELISA Kit (AB253229)

Example of mouse MAP2 standard curve in 1X Cell Extraction Buffer PTR.

The MAP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma, Tissue Extracts

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 43.31 pg/mL

検出範囲

156.25 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse MAP2 ELISA Kit (ab253229) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MAP2 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MAP2 with 43.31 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

MAP2 (Microtubule-associated protein 2) is a neuronal protein that regulates the structure and stability of microtubules. It belongs to the MAP2/Tau family. Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. Mouse MAP2 shares 80.2%, 92.3%, and 74.4% sequence homology with human, rat, and bovine, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "90 - 99 %", "average": "= 93" }, { "sample": "Serum", "range": "82 - 87 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "82 - 89 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "93 - 101 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "81 - 86 %", "average": "= 84" }, { "sample": "Tissue Extracts", "range": "80 - 83 %", "average": "= 81" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253229-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253229-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253229-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253229-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253229-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253229-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253229-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253229-CMP12", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BR", "number":"AB253229-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MAP2 Detector Antibody", "number":"AB253229-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253229-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse MAP2 Capture Antibody", "number":"AB253229-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse MAP2 Lyophilized Recombinant Protein", "number":"AB253229-CMP05", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

See full target information Map2

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadd2671 PubMed36867706

2023

CREB3L2-ATF4 heterodimerization defines a transcriptional hub of Alzheimer's disease gene expression linked to neuropathology.

Applications

Unspecified application

Species

Unspecified reactive species

Cláudio Gouveia Roque,Kyung Min Chung,Ethan P McCurdy,Radhika Jagannathan,Lisa K Randolph,Krystal Herline-Killian,Jimena Baleriola,Ulrich Hengst

View all publications

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websiteProtocolBooklet

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Mouse MAP2 ELISA Kit