AB207617

Mouse Legumain ELISA Kit

Recombinant
SimpleStep
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Mouse Legumain ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse Legumain with a sensitivity of 8.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Prsc1, Lgmn, Legumain

5 Images

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

sELISA

Supplier Data

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

Interpolated concentrations of native Legumain in mouse serum and spiked Legumain in RPMI Base Media.

The concentrations of Legumain were measured in duplicates, interpolated from the Legumain standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, and RPMI Base Media 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 9.1 ng/mL in serum and 1.7 ng/mL in RPMI Base Media 10%.

sELISA

Supplier Data

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

Interpolated concentrations of native Legumain in mouse RAW 264.7 cell extract based on a 600 µg/mL extract load.

The concentrations of Legumain were measured in duplicate and interpolated from the Legumain standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 2.77 ng/mL in mouse RAW 264.7 cell extract.

sELISA

Supplier Data

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

Interpolated concentrations of native Legumain in mouse plasma (heparin, EDTA, and citrate).

The concentrations of Legumain were measured in duplicates, interpolated from the Legumain standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (heparin) 5%, plasma (EDTA) 2.5% and plasma (citrate) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Legumain concentration was determined to be 14.1 ng/mL in plasma (heparin), 7.7 ng/mL in plasma (EDTA) and 6.5 ng/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

Example of mouse Legumain standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Legumain ELISA Kit (AB207617)

Example of mouse Legumain standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 8.2 pg/mL

検出範囲

54.69 - 3500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Legumain ELISA Kit (ab207617) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Legumain protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Legumain with NULL NULL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Legumain is a lysosomal cysteine protease that plays a pivotal role in the endosomal/lysosomal degradation system. Specifically, Legumain is required for the normal degradation of internalized EGFR, allowing Legumain to regulate cell proliferation via this role. Mouse Legumain has 94% and 83% identity to rat and human Legumain, respectively.Legumain has a strong specificity for hydrolysis of asparaginyl bonds, but can also cleave aspartyl bonds slowly, especially under acidic conditions. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "RAW 264.7", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6" }, { "reproducibilityType": "Intra", "sample": "RAW 264.7", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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回収率

[ { "sample": "Serum", "range": "108 - 120 %", "average": "= 113" }, { "sample": "EDTA Plasma", "range": "107 - 112 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "111 - 115 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "107 - 116 %", "average": "= 112" }, { "sample": "Cell culture media", "range": "97 - 101 %", "average": "= 99" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Legumain also known as asparaginyl endopeptidase is an enzyme that plays a mechanical role as a protease. This enzyme has a molecular mass of approximately 56 kDa and it functions by catalyzing the hydrolysis of asparagine residues. Legumain is expressed in various tissues with higher levels observed in kidneys placenta and liver. Its proteolytic activity is essential for the breakdown of intracellular proteins within lysosomes and endosomal compartments.

Biological function summary

Legumain contributes to protein degradation and antigen processing. It does not operate alone as it is part of larger protein complexes which enhance its stability and function. Through its role in proteolysis legumain influences cellular processes like maturation of endolysosomal enzymes and processing of proenzymes. Additionally it assists in activating other proteases signifying its critical position in maintaining cellular proteostasis.

Pathways

Legumain participates in the regulation of immune responses and cellular homeostasis. It plays a significant role in the lysosomal pathway where it partners with cathepsins to assist in the degradation of proteins. Additionally legumain influences the MHC class II pathway by contributing to the cleavage of invariant chain aiding antigen presentation. Its interaction with cathepsins and the impact on antigen processing indicates its necessity in immune system functionality.

Legumain has been linked to disorders such as cancer and neurodegeneration. In cancer elevated legumain levels correlate with tumor invasiveness and metastasis especially in association with proteins like matrix metalloproteinases. Meanwhile in neurodegenerative conditions its dysregulation may contribute to abnormal protein accumulation and synaptic dysfunction. Understanding legumain's role and interactions can guide therapeutic strategies for these diseases.

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製品プロトコール

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ターゲットの情報

Has a strict specificity for hydrolysis of asparaginyl bonds (PubMed : 24407422, PubMed : 9742219, PubMed : 9891971). Can also cleave aspartyl bonds slowly, especially under acidic conditions (PubMed : 24407422, PubMed : 9742219, PubMed : 9891971). Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system (By similarity). Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol (PubMed : 37347855). Required for normal lysosomal protein degradation in renal proximal tubules (PubMed : 17350006, PubMed : 21292981). Required for normal degradation of internalized EGFR (PubMed : 21292981). Plays a role in the regulation of cell proliferation via its role in EGFR degradation (PubMed : 21292981).

See full target information Lgmn

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 11:1014 PubMed33243972

2020

Legumain is a predictor of all-cause mortality and potential therapeutic target in acute myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Yuhu He,Pu Zou,Yilei Hu,Xuping Li,Liang Tang,Zhaowei Zhu,Shi Tai,Tao Tu,Yichao Xiao,Mingxian Chen,Chenlu Wu,Shenghua Zhou

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Mouse Legumain ELISA Kit