Mouse Interferon gamma ELISA Set (without plates) (ab47618)
製品の概要
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製品名
Mouse Interferon gamma ELISA Set (without plates)
Interferon gamma キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 15 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IFNγ Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IFNγ in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IFN-gamma ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IFN-gamma in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine Interferon gamma Range: 31.25 pg/ml - 1000 pg/ml Sensitivity < 15 pg/ml.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 15 x 96 tests 5 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 6 vials 2 vials 4 vials Detection biotinylated anti-Murine IFN gamma antibody 3 vials 1 vial 2 vials IFN gamma Standard (Lyophilized) Yellow 15 vials 5 vials 10 vials Murine IFN gamma Capture Antibody 3 x 0.25ml 1 x 0.25ml 2 x 0.25ml Streptavidin-HRP 3 vials 1 vial 2 vials -
研究分野
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機能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
組織特異性
Released primarily from activated T lymphocytes. -
関連疾患
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
配列類似性
Belongs to the type II (or gamma) interferon family. -
翻訳後修飾
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IF 1
- IFG
- IFI
see all -
参照データベース
- Entrez Gene: 15978 Mouse
- SwissProt: P01580 Mouse
- Unigene: 240327 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47618の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Murine Interferon gamma ELISA Set (ab47618)Murine IFN-gamma Standard curve ranging from 31.25 to 1000 pg/mL
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47618 は論文での使用が確認できていません。