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AB222503

Mouse IL-6 ELISA Kit

Mouse IL-6 ELISA Kit

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(2 Reviews)

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(192 Publications)

Mouse IL-6 ELISA kit is a single-wash 90-min SimpleStep ELISA® used to quantification of mouse interleukin-6 (IL-6) with a sensitivity of 11.3 pg/mL. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA - 450nm readout; works on any plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well for higher throughput
- Broad sample compatibility - works with serum, plasma, and cell culture supernatants
- Cited in over 130 publications

別名を表示する

Il-6, Il6, Interleukin-6, IL-6, B-cell hybridoma growth factor, Interleukin HP-1

9 Images
Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve.

The IL-6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Functional Studies - Mouse IL-6 ELISA Kit (AB222503)
  • FuncS

Lab

Functional Studies - Mouse IL-6 ELISA Kit (AB222503)

IL-6 secretion in NIH/3T3 cells following stimulation with IL-17A in the presence of either an anti-IL17RA antibody (ab327248) or an IgG isotype control (ab199376). (A) Dose–response curve of NIH/3T3 cells exposed to increasing concentrations of IL-17A. (B) IL-6 secretion induced by 1000 ng/mL mouse IL-17A following treatment with anti-IL17RA antibody(circles, solid line) or IgG isotype control (triangles, dotted line) across a range of concentrations. The IC₅₀ for inhibition of IL-6 secretion is 16.24 µg/mL. NIH/3T3 cells were seeded at 1 × 10⁴cells per well in a 96-well plate. Cells were pre-incubated with a dilution series of anti-IL17RA antibody for 6 hours prior to IL-17A stimulation and then cultured for 48 hours in medium containing 10% FBS. IL-6 concentrations were measured using a Mouse IL-6 ELISA Kit (Abcam, ab222503).

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse serum and plasma samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 39 pg/mL in serum, 42 pg/mL in plasma (heparin), 43 pg/mL in plasma (EDTA), and 32 pg/mL in plasma (citrate).

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of spiked IL-6 in mouse serum and plasma samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (heparin) 50%, plasma (EDTA) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution.

Native IL-6 was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Recombinant IL-6 was spiked into the following biological samples and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Interpolated concentrations of native IL-6 in mouse cell culture supernatant samples.

The concentrations of IL-6 were measured in duplicates, interpolated from the IL-6 standard curves and corrected for sample dilution. Undiluted samples are as follows : stimulated RAW264.7 cell culture supernatant 1 : 50 and stimulated spleen cell culture supernatant 1 : 16. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-6 concentration was determined to be 26,793 pg/mL in stimulated RAW264.7 cell culture supernatant, and 15,127 pg/mL in stimulated spleen cell culture supernatant.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

Example of mouse IL-6 standard curve. The IL-6 standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)
  • sELISA

Lab

Sandwich ELISA - Mouse IL-6 ELISA Kit (AB222503)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative
検出感度

= 11.3 pg/mL

検出範囲

15.6 - 1000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

製品の詳細

Mouse IL-6 ELISA Kit ab222503 is a rapid single-wash 90-min ELISA kit to measure cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 11.3 pg/mL.

How the assay works

SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant mouse IL-6 protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit.

Mouse IL-6 ELISA Kit ab222503 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse IL-6 ELISA kit ab222503

Mouse IL-6 ELISA kit ab222503 has been used to assess inflammation in different physio-pathological contexts such as liver disease(1), diabetes(2), and aging(3).

References:

(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Lili Shi et al. 2024, PMID: 38169591,
(3)Yang Bao et al. 2023, PMID: 37954634

Save your precious samples

Our 384-well Mouse IL-6 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Mouse IL-6 Antibody Pair - BSA and Azide free ab241663
- Anti-IL-6 antibody [EPR16610-380] - BSA and Azide free (Capture) ab242490
- Anti-IL-6 antibody [EPR16610-318] - BSA and Azide free (Detector) ab242739

Related and recommended products

Mouse IL-6 ELISA kit ab222503 is commonly used to measure IL-6 as a marker of inflammation.

Other ELISA kits to measure inflammatory markers include:

- Mouse TNF alpha ELISA kit ab208348
- Mouse IL-10 ELISA kit ab255729
- Mouse IL-1 beta ELISA kit ab197742

In glioblastoma, IL-6 is known to activate several pro-oncogenic signaling pathways.
Explore our comprehensive range of glioblastoma research solutions here: https://www.abcam.com/en-us/products/product-recommendations/glioblastoma-research-tools

In Alzheimer's disease, increased levels of IL-6 in the plasma or cerebrospinal fluid of patients is associated with cognitive impairment.
Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools

Interleukin 6 (IL-6) is a cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells. IL-6 is involved in lymphocyte and monocyte differentiation and IL-6 induces myeloma and plasmacytoma growth as well as nerve cells differentiation. B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS are all responsive to IL-6. IL-6 is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance

精度

[ { "reproducibilityType": "Inter", "sample": "Spleen CCS", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" }, { "reproducibilityType": "Intra", "sample": "Spleen CCS", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

回収率

[ { "sample": "Serum", "range": "103 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "109 - 116 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "95 - 103 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "96 - 102 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "89 - 112 %", "average": "= 100" } ]

製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

製品プロトコール

ターゲットの情報

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism (Probable). Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (PubMed : 8910279). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (PubMed : 27893700).. IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury. In the adaptive immune response, is required for the differentiation of B-cells into immunoglolin-secreting cells (Probable). Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Together with IL21, controls the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). Required to drive naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed : 16990136, PubMed : 27893700). Also required for proliferation of myeloma cells and the survival of plasmablast cells (Probable).. Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability. Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed : 17075861). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed : 11786910). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed : 28402851). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (PubMed : 11113088). Also acts as a myokine (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed : 11113088, PubMed : 8910279). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed : 15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).
See full target information Il6

文献 (192)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 32: PubMed40539450

2025

Protective effects of lipid mediators, obtained from docosahexaenoic acid via soybean lipoxygenase, on lipopolysaccharide‑induced acute lung injury through the NF‑κB and Nrf2/HO‑1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Su,Hack Sun Choi,Soon Kyu Kwon,Yunjon Han,Soon-Chang Cho,Jin Hyuk Shin,Yong-Suk Jang,Jong Hyun Choi,Jeong-Woo Seo

Journal of cell communication and signaling 19:e70019 PubMed40520073

2025

Role of circ_0012856 in modulating molecular pathways of diabetic peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Chen,Fan Zhang,Yangyuxi Chen,Yingqing Lu,Xinxin Liu,Yuanzhang Tang,Fengrui Yang

Naunyn-Schmiedeberg's archives of pharmacology : PubMed40514518

2025

Alpha-asarone relieves nasal inflammation, epithelial barrier damage, and mitochondrial damage in allergic rhinitis by inhibiting mitochondrial ROS via the SIRT1/PGC-1α pathway.

Applications

Unspecified application

Species

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Bin Xu,Weimin Gao,Haitong Li,Xiaojuan Cao,Xiaohong Chen,Nannan Dong,Lingfang Wu,Yunzhen Luo

Theranostics 15:6022-6043 PubMed40365283

2025

pH-responsive nano-vaccine combined with anti-PD-1 antibodies for enhanced immunotherapy of breast cancer.

Applications

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Species

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Ning Wang,Hong Yu,Jianqiao Yin,Xiaopeng Yu

PloS one 20:e0322334 PubMed40323903

2025

Human cytomegalovirus-IE2 suppresses antigen presentation of macrophage through the IL10/STAT3 signalling pathway in transgenic mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjuan Zhang,Qing Wang,Shuo Han,Guanghui Song,Bin Wang,Yunyang Wang

Nature metabolism 7:823-841 PubMed40275022

2025

Hepatic gluconeogenesis and PDK3 upregulation drive cancer cachexia in flies and mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Ezequiel Dantas,Miriam Ferrer,Ting Miao,Mujeeb Qadiri,Yifang Liu,Aram Comjean,Emma E Davidson,Tiffany Perrier,Tanvir Ahmed,Yanhui Hu,Marcus D Goncalves,Tobias Janowitz,Norbert Perrimon

Vaccines 13: PubMed40266088

2025

Immunogenicity of Rabies Virus G-Protein mRNA Formulated with Muscle-Targeting Lipid Nanoparticles in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Li,Huarong Bai,Xueliang Yu,Qiang Liu,Rongkuan Hu

Archivum immunologiae et therapiae experimentalis 73: PubMed40098542

2025

Isorhynchophylline Inhibits NLRP3 Inflammasome and Improves Gestational Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Li Pu,Li Chen,Kun Yu,Yueming Zhang,Caifeng Wang,Feizhou Jiang,Jia Shi,Jingjing Meng

Cell biology and toxicology 41:59 PubMed40080209

2025

Exosome-mediated effects of BRCA1 on cardiovascular artery disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hairui Yu,Dong Wei,Weiqian Liao,Xiaoming Shang,Dandan Li,Chunzhao Liu,Qimei Deng,Haiquan Huangfu

Journal of thoracic disease 17:623-640 PubMed40083491

2025

Novel therapeutic strategy: Nrf2 activation in targeting senescence-related changes in chronic obstructive pulmonary disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fenhua Jin,Hui Lin,Shufang Pan
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