AB208989

Mouse IL-28A ELISA Kit

Recombinant
SimpleStep
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Mouse IL-28A ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-28A with a sensitivity of 2.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Il28a, Ifnl2, Interferon lambda-2, IFN-lambda-2, Interleukin-28A, IL-28A

3 Images

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Interpolated concentrations of spiked IL-28A in mouse serum and plasma.

The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : serum 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Interpolated concentrations of spiked IL-28A in cell culture supernatant samples.

The concentrations of IL-28A were measured in duplicates, interpolated from the (target) standard curves and corrected for sample dilution. 1X diluted samples are as follows : lung cell culture supernatant 25%, and RAW 264.7 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-28A ELISA Kit (AB208989)

Example of mouse IL-28A standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 2.4 pg/mL

検出範囲

15.63 - 1000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse IL-28A ELISA Kit (ab208989) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-28A protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IL-28A with 2.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IL-28A (Interleukin 28A), also known as IFN-lambda 2 (Interferon-lambda 2), is a class II cytokine receptor ligand with antiviral, antitumor, and immunomodulatory activities.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "102 - 107 %", "average": "= 105" }, { "sample": "Serum", "range": "110 - 112 %", "average": "= 111" }, { "sample": "EDTA Plasma", "range": "106 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "109 - 112 %", "average": "= 111" }, { "sample": "Cell culture media", "range": "106 - 108 %", "average": "= 108" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets : is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.

See full target information Ifnl2

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websiteProtocolBooklet

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Mouse IL-28A ELISA Kit