Mouse IL-2 ELISA Set (without plates) (ab47591)
製品の概要
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製品名
Mouse IL-2 ELISA Set (without plates)
IL-2 キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 5 pg/ml -
検出範囲
15.6 pg/ml - 500 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-2 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure murine IL-2 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
The murine IL-2 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant Murine IL-2
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 10 x 96 tests 5 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 4 vials 2 vials 6 vials Detection biotinylated anti-murine IL-2 antibody 2 x 0.55ml 1 x 0.55ml 3 x 0.55ml Murine IL-2 Capture antibody 2 x 0.1ml 1 x 0.1ml 3 x 0.1ml Murine IL-2 standard (Lyophilized) Yellow 10 vials 5 vials 15 vials Streptavidin-HRP 2 vials 1 vial 3 vials -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47591の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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IL-2 Murine ELISA Set (ab47591)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab47591 は 2 報の論文で使用されています。
- Li S et al. CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer. J Gastroenterol Hepatol 37:154-163 (2022). PubMed: 34734434
- Zhang L et al. c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis. Clin Transl Med 11:e464 (2021). PubMed: 34586738