AB47591

Mouse IL-2 ELISA Set (without plates)

Name: Mouse IL-2 ELISA Set (without plates) (ab47591) | Abcam
Brand: Abcam Limited
SKU: AB47591
Price: 155000 JPY
Availability: InStock

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(2 Publications)

Mouse IL-2 ELISA Set (without plates) is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-2 ELISA Set (without plates) in Mouse in Biofluids, Cell Culture Media samples.

別名を表示する

Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

1 Images

Sandwich ELISA - Mouse IL-2 ELISA Set (without plates) (AB47591)

sELISA

Unknown

Sandwich ELISA - Mouse IL-2 ELISA Set (without plates) (AB47591)

Key facts

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Mouse

アッセイタイプ

Sandwich (quantitative)

検出感度

< 5 pg/mL

検出範囲

15.6 - 500 pg/mL

アッセイ時間

3h 45m

アッセイプラットフォーム

Reagents

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's IL-2 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-2 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

This ELISA Set is intended to measure murine IL-2 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

The murine IL-2 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

Recognizes both natural and recombinant Murine IL-2

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製品内容

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab47591-5x96Tests", "assetComponentDetails": [ { "size":"1 x 0.1 mL", "name":"Murine IL-2 Capture antibody", "number":"AB47591-CMP01" }, { "size":"2 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47591-CMP04" }, { "size":"1 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47591-CMP02" }, { "size":"5 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB47591-CMP03" }, { "size":"1 x 0.55 mL", "name":"Detection biotinylated anti-murine IL-2 antibody", "number":"AB47591-CMP05" } ] }, "15x96Tests": { "sellingSize": "15 x 96 Tests", "publicAssetCode":"ab47591-15x96Tests", "assetComponentDetails": [ { "size":"6 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47591-CMP04" }, { "size":"15 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB47591-CMP03" }, { "size":"3 x 0.1 mL", "name":"Murine IL-2 Capture antibody", "number":"AB47591-CMP01" }, { "size":"3 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47591-CMP02" }, { "size":"3 x 0.55 mL", "name":"Detection biotinylated anti-murine IL-2 antibody", "number":"AB47591-CMP05" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab47591-10x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Streptavidin-HRP", "number":"AB47591-CMP02", "productcode":"" }, { "size":"2 x 0.1 mL", "name":"Murine IL-2 Capture antibody", "number":"AB47591-CMP01", "productcode":"" }, { "size":"4 x 1 Vial", "name":"Chromogen TMB Substrate Solution", "number":"AB47591-CMP04", "productcode":"" }, { "size":"10 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB47591-CMP03", "productcode":"" }, { "size":"2 x 0.55 mL", "name":"Detection biotinylated anti-murine IL-2 antibody", "number":"AB47591-CMP05", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 14614860, PubMed : 9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 14614860, PubMed : 27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed : 14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed : 9814585).

See full target information Il2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 37:154-163 PubMed34734434

2021

CD155/TIGIT signaling regulates the effector function of tumor-infiltrating CD8+ T cell by NF-κB pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Li,Jihu Ding,Ying Wang,Xianling Wang,Lin Lv

Clinical and translational medicine 11:e464 PubMed34586738

2021

c-Myb facilitates immune escape of esophageal adenocarcinoma cells through the miR-145-5p/SPOP/PD-L1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Zhang,Xiaohui Wang,Yunfei Li,Jing Han,Xianzheng Gao,Shenglei Li,Feng Wang

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Mouse IL-2 ELISA Set (without plates)