Mouse IL-2 ELISA Kit (ab46096)
Key features and details
- Sensitivity: 3.4 pg/ml
- Range: 15.6 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-2 ELISA Kit
IL-2 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% A 10 170pg/ml 13 7.8% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% B 8 137pg/ml 8.9 6.4% -
サンプルの種類
Cell culture supernatant, Serum -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 3.4 pg/ml -
検出範囲
15.6 pg/ml - 500 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-2 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.
A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.
This kit will recognize both endogenous and recombinant mouse IL-2.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 12ml 1 x 23ml IL-2 Biotinylated Detection antibody 1 x 400µl 2 x 400µl IL-2 Microplate (12 x 8 well strips) 1 unit 2 units Murine IL-2 standard (Lyophilized) Yellow 2 vials 4 vials Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究分野
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機能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
関連疾患
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
配列類似性
Belongs to the IL-2 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Aldesleukin
- IL 2
- IL-2
see all -
参照データベース
- Entrez Gene: 16183 Mouse
- SwissProt: P04351 Mouse
- Unigene: 14190 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46096の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab46096.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab46096 は 3 報の論文で使用されています。
- Hadad SE et al. In vivo evidence: Repression of mucosal immune responses in mice with colon cancer following sustained administration of Streptococcus thermophiles. Saudi J Biol Sci 28:4751-4761 (2021). PubMed: 34354463
- Safhi MM Nephroprotective Effect of Zingerone against CCl4-Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism. Oxid Med Cell Longev 2018:2474831 (2018). PubMed: 29636837
- Zamecnik CR et al. Injectable Polymeric Cytokine-Binding Nanowires Are Effective Tissue-Specific Immunomodulators. ACS Nano 11:11433-11440 (2017). ELISA ; Mouse . PubMed: 29124929