AB46096

Mouse IL-2 ELISA Kit

Name: Mouse IL-2 ELISA Kit (ab46096) | Abcam
Brand: Abcam Limited
SKU: AB46096
Price: 109000 JPY
Availability: InStock

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(3 Publications)

Mouse IL-2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse IL-2 in Mouse in Cell Culture Media, Biofluids samples.

別名を表示する

Il-2, Il2, Interleukin-2, IL-2, T-cell growth factor, TCGF

1 Images

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB46096)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-2 ELISA Kit (AB46096)

Representative Standard Curve using ab46096.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture supernatant, Serum

交差種

Mouse

アッセイタイプ

Sandwich (quantitative)

検出感度

< 3.4 pg/mL

検出範囲

15.6 - 500 pg/mL

アッセイ時間

3h 45m

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's IL-2 mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-2 in mouse serum, buffered solutions and cell culture medium.

A monoclonal antibody specific for IL-2 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-2 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-2 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-2 present in the samples.

This kit will recognize both endogenous and recombinant mouse IL-2.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 8, "mean": "137 pg/mL", "standardDeviation": "8.9", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 10, "mean": "170 pg/mL", "standardDeviation": "13", "coefficientOfVariability": "7.8" } ]

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製品内容

{ "values": { "2x96Tests": { "sellingSize": "2 x 96 Tests", "publicAssetCode":"ab46096-2x96Tests", "assetComponentDetails": [ { "size":"1 x 23 mL", "name":"HRP Diluent", "number":"AB46096-CMP03" }, { "size":"2 x 10 mL", "name":"200X Wash Buffer", "number":"AB46096-CMP05" }, { "size":"1 x 25 mL", "name":"10X Standard Diluent Buffer", "number":"AB46096-CMP07" }, { "size":"1 x 24 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46096-CMP09" }, { "size":"4 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB46096-CMP06" }, { "size":"4 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46096-CMP04" }, { "size":"1 x 13 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46096-CMP01" }, { "size":"2 x 11 mL", "name":"Stop Reagent", "number":"AB46096-CMP02" }, { "size":"2 x 400 µL", "name":"IL-2 Biotinylated Detection antibody", "number":"AB46096-CMP08" }, { "size":"2 x 1 Unit", "name":"IL-2 Microplate (12 x 8 well strips)", "number":"AB46096-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab46096-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"HRP Diluent", "number":"AB46096-CMP03", "productcode":"" }, { "size":"1 x 11 mL", "name":"Chromogen TMB Substrate Solution", "number":"AB46096-CMP09", "productcode":"" }, { "size":"1 x 7.5 mL", "name":"Biotinylated Antibody Diluent", "number":"AB46096-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Murine IL-2 standard (Lyophilized)", "number":"AB46096-CMP06", "productcode":"" }, { "size":"2 x 5 µL", "name":"Streptavidin-HRP", "number":"AB46096-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Reagent", "number":"AB46096-CMP02", "productcode":"" }, { "size":"1 x 400 µL", "name":"IL-2 Biotinylated Detection antibody", "number":"AB46096-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"10X Standard Diluent Buffer", "number":"AB46096-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-2 Microplate (12 x 8 well strips)", "number":"AB46096-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"200X Wash Buffer", "number":"AB46096-CMP05", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed : 14614860, PubMed : 9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed : 14614860, PubMed : 27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed : 14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed : 9814585).

See full target information Il2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Saudi journal of biological sciences 28:4751-4761 PubMed34354463

2021

evidence: Repression of mucosal immune responses in mice with colon cancer following sustained administration of .

Applications

Unspecified application

Species

Unspecified reactive species

Sahar El Hadad,Maha Alsolami,Alia Aldahlawi,Jehan Alrahimi,Fatemah Basingab,Shahira Hassoubah,Hani Alothaid

Oxidative medicine and cellular longevity 2018:2474831 PubMed29636837

2018

Nephroprotective Effect of Zingerone against CCl-Induced Renal Toxicity in Swiss Albino Mice: Molecular Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed M Safhi

ACS nano 11:11433-11440 PubMed29124929

2017

Injectable Polymeric Cytokine-Binding Nanowires Are Effective Tissue-Specific Immunomodulators.

Applications

ELISA

Species

Mouse

Colin R Zamecnik,Margaret M Lowe,David M Patterson,Michael D Rosenblum,Tejal A Desai

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Mouse IL-2 ELISA Kit