AB254509

Mouse IL-18BP ELISA Kit

Name: Mouse IL-18BP ELISA Kit (ab254509) | Abcam
Brand: Abcam Limited
SKU: AB254509
Price: 109000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Recombinant
SimpleStep
詳細を見る

(1 Review)

(1 Publication)

Mouse IL-18BP ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse IL-18BP with a sensitivity of 151.82 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Igifbp, Il18bp, Interleukin-18-binding protein, IL-18BP, Interferon gamma-inducing factor-binding protein

3 Images

ELISA - Mouse IL-18BP ELISA Kit (AB254509)

ELISA

Supplier Data

ELISA - Mouse IL-18BP ELISA Kit (AB254509)

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18BP ELISA Kit (AB254509)

Example of mouse IL-18BP standard curve in Sample Diluent NS.

The IL-18BP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse IL-18BP ELISA Kit (AB254509)

Interpolated concentrations of native IL-18BP in mouse serum and plasma (citrate) samples.

The concentrations of IL-18BP were measured in duplicates, interpolated from the IL-18BP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5% and plasma (citrate) 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18BP concentration was determined to 175.87 ng/mL in serum and 201.01 ng/mL in plasma (citrate).

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Serum

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 151.82 pg/mL

検出範囲

468.75 - 30000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Mouse IL-18BP ELISA Kit ab254509 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse IL-18BP in citrate plasma, serum. This SimpleStep sensitivity is 151.82 pg/mL.

How the assay works

Mouse IL-18BP SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse IL-18BP ELISA Kit ab254509 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IL-18BP antibody [EPR22368-264] - BSA and Azide free (Capture) ab259730
- Anti-IL-18BP antibody [EPR22368-254] - BSA and Azide free (Detector) ab259730

IL-18BP is a protein that binds to IL-18, preventing it from interacting with its receptor. IL-18BP inhibits the early TH1 cytokine response. IL-18 is produced constitutively by mononuclear cells.

style

left-column

精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

style

left-column

回収率

[ { "sample": "Serum", "range": null, "average": "= 97" }, { "sample": "Citrate plasma", "range": null, "average": "= 98" } ]

style

left-column

製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab254509-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB254509-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB254509-CMP05" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254509-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB254509-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Mouse IL-18BP Capture Antibody", "number":"AB254509-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254509-CMP03" }, { "size":"2 x 10 Vial", "name":"Mouse IL-18BP Lyophilized Recombinant Protein", "number":"AB254509-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB254509-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254509-CMP04" }, { "size":"1 x 6000 µL", "name":"10X Mouse IL-18BP Detector Antibody", "number":"AB254509-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab254509-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB254509-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB254509-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB254509-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB254509-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB254509-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB254509-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB254509-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse IL-18BP Lyophilized Recombinant Protein", "number":"AB254509-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse IL-18BP Capture Antibody", "number":"AB254509-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"10X Mouse IL-18BP Detector Antibody", "number":"AB254509-CMP08", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response (By similarity).

See full target information Il18bp

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:356-371 PubMed39196121

2024

Kupffer cells dictate hepatic responses to the atherogenic dyslipidemic insult.

Applications

Unspecified application

Species

Unspecified reactive species

Giada Di Nunzio,Sanna Hellberg,Yuyang Zhang,Osman Ahmed,Jiawen Wang,Xueming Zhang,Hanna M Björck,Veronika Chizh,Ruby Schipper,Hanna Aulin,Roy Francis,Linn Fagerberg,Anton Gisterå,Jari Metso,Valentina Manfé,Anders Franco-Cereceda,Per Eriksson,Matti Jauhiainen,Carolina E Hagberg,Peder S Olofsson,Stephen G Malin

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse IL-18BP ELISA Kit