AB326908
Mouse IL-15 ELISA Kit, Chemiluminescent
Mouse IL-15 ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
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Mouse IL-15 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse IL-15 with higher sensitivity and broader measuring range.
- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 16 - 35000 pg/ml with a sensitivity of 7.1 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
別名を表示する
Interleukin-15, IL-15, Il15
3 Images
- sELISA
Lab
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Dynamic range comparison between the Chemiluminescent Mouse IL-15 SimpleStep ELISA, colorimetric Mouse IL-15 SimpleStep ELISA (ab275898), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows comparable sensitivity and increased dynamic range.
- sELISA
Lab
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Standard curve comparison between the Chemiluminescent Mouse IL-15 SimpleStep ELISA and colorimetric Mouse IL-15 SimpleStep ELISA (ab275898) in Sample Diluent NS + 1X Enhancer. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse IL-15 ELISA Kit, Chemiluminescent (AB326908)
Interpolated concentrations of spike IL-15 in mouse serum and plasma samples
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
製品の詳細
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) is a rapid single-wash sandwich 55-min ELISA to measure Mouse IL-15 in Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 7.1 pg/mL.
How the assay works
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-15 (ab326908) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-15 antibody [EPR22635-211] - BSA and Aside free (Capture) ab276163
- Anti-IL-15 antibody [EPR22635-101] - BSA and Aside free (Detector) ab276164
How the assay works
Mouse IL-15 ELISA Kit, Chemiluminescent (ab326908) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse IL-15 (ab326908) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-IL-15 antibody [EPR22635-211] - BSA and Aside free (Capture) ab276163
- Anti-IL-15 antibody [EPR22635-101] - BSA and Aside free (Detector) ab276164
精度
[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.53" } ]
回収率
[ { "sample": "Serum", "range": "80 - 84 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "79 - 86 %", "average": "= 86" }, { "sample": "Cell culture media", "range": "107 - 108 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "100 - 106 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "79 - 81 %", "average": "= 81" } ]
製品内容
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出荷温度及び保存条件
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
ターゲットの情報
Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems (PubMed : 11466332, PubMed : 8757333). Stimulates the proliferation and activation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines (PubMed : 8757333). In monocytes, induces the production of IL8 and monocyte chemotactic protein 1/CCL2, two chemokines that attract neutrophils and monocytes respectively to sites of infection (By similarity). Unlike most cytokines, which are secreted in soluble form, IL15 is expressed in association with its high affinity IL15RA on the surface of IL15-producing cells and delivers signals to target cells that express IL2RB and IL2RG receptor subunits. Binding to its receptor triggers the phosphorylation of JAK1 and JAK3 and the recruitment and subsequent phosphorylation of signal transducer and activator of transcription-3/STAT3 and STAT5. In mast cells, induces the rapid tyrosine phosphorylation of STAT6 and thereby controls mast cell survival and release of cytokines such as IL4 (PubMed : 10882748, PubMed : 19632221).
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