Mouse IL-12 p70 ELISA Kit

製品の詳細

Mouse IL-12 p70 ELISA Kit ab119531 is a sandwich ELISA to measure Mouse IL-12 p70 in serum, cell culture supernatant with a sensitivity of 10 pg/ml.

How the assay works

IL-12 p70 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with a Biotin-conjugated anti-mouse IL-12 p70 detection antibody and the microplate is then incubated at room temperature. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-12 p70 captured on the plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Mouse IL-12 p70 ELISA Kit ab119531 protocol summary

1. Add standard or sample to appropriate wells. Incubate the plate
2. Wash and add Biotinylated mouse anti-TGF beta 1 monoclonal antibody to appropriate wells. Incubate the plate.
3. Wash and add prepared Streptavidin-HRP Conjugate to appropriate wells. Incubate at room temperature
4. Wash and add TMB Substrate to each well
5. Add Stop Solution to each well. Read immediately

The standard is affinity purified.