AB100698

Mouse IL-11 ELISA Kit

Name: Mouse IL-11 ELISA Kit (ab100698) | Abcam
Brand: Abcam Limited
SKU: AB100698
Price: 109000 JPY
Availability: InStock

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(1 Publication)

Mouse IL-11 ELISA Kit is a Sandwich ELISA for the measurement of Mouse IL-11 in Mouse in Biofluids, Cell Culture Media samples.

別名を表示する

Interleukin-11, IL-11, Il11, IL 11, Interleukin 11

1 Images

ELISA - Mouse IL-11 ELISA Kit (AB100698)

ELISA

Supplier Data

ELISA - Mouse IL-11 ELISA Kit (AB100698)

Representative standard curve using ab100698.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

< 1 ng/mL

検出範囲

1.85 - 450 ng/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's IL-11 Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-11 in plasma and cell culture supernatants. (Mouse IL-11 concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for mouse IL-11 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-11 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-11 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-11 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect the endogenous Mouse IL-11 in normal serum with ab100698, only in serum spiked with Mouse IL-11.

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回収率

[ { "sample": "Cell culture supernatant", "range": "70 - 87 %", "average": "= 77.35" }, { "sample": "Plasma", "range": "95 - 113 %", "average": "= 117.3" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100698-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100698-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100698-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100698-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL-11 Microplate (12 x 8 wells)", "number":"AB100698-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100698-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100698-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Mouse IL-11 Standard (lyophilized)", "number":"AB100698-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-mouse IL-11", "number":"AB100698-CMP09", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100698-CMP02", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed : 8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed : 22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed : 10026196, PubMed : 23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed : 22253262, PubMed : 23948300). The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling' (By similarity).

See full target information Il11

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 316:G251-G262 PubMed30520693

2018

Overexpression of IL-11 promotes premalignant gastric epithelial hyperplasia in isolation from germline gp130-JAK-STAT driver mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Jon N Buzzelli,Louise O'Connor,Michelle Scurr,Sharleen Chung Nien Chin,Angelique Catubig,Garrett Z Ng,Masanobu Oshima,Hiroko Oshima,Andrew S Giraud,Philip Sutton,Louise M Judd,Trevelyan R Menheniott

View all publications

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websiteProtocolBooklet

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Mouse IL-11 ELISA Kit