Mouse IL-10 ELISA Set (without plates) (ab47599)
製品の概要
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製品名
Mouse IL-10 ELISA Set (without plates)
IL-10 キット 製品一覧 -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
31.25 pg/ml - 1000 pg/ml -
全工程の試験時間
3h 45m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-10 Murine in vitro ELISA Set is designed for the quantitative measurement of Murine IL-10 in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
The murine IL-10 ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
This ELISA Set is intended to measure murine IL-10 in cell -culture supernatants, as well as in serum samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
Recognizes both natural and recombinant Murine IL-10
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Reagents
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 5 x 96 tests 15 x 96 tests 10 x 96 tests Chromogen TMB Substrate Solution 2 vials 6 vials 4 vials Detection biotinylated anti-murine IL-10 antibody 1 x 0.55ml 3 x 0.55ml 2 x 0.55ml Murine IL-10 Capture antibody 1 x 0.5ml 3 x 0.5ml 2 x 0.5ml Murine IL-10 standard 5 vials 15 vials 10 vials Streptavidin-HRP 1 vial 3 vials 2 vials -
研究分野
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CSIF
- Cytokine synthesis inhibitory factor
- IL 10
see all -
参照データベース
- Entrez Gene: 16153 Mouse
- SwissProt: Q0VBJ1 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47599の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Murine IL-10 ELISA Set (ab47599)Murine IL-10 standard curve ranging from 31.25 to 1000 pg/mL
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab47599 は論文での使用が確認できていません。