Mouse IL-10 ELISA kit (ab108870)
Key features and details
- Sensitivity: 0.014 ng/ml
- Range: 0.125 ng/ml - 1 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IL-10 ELISA kit
IL-10 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.9% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Sandwich (quantitative) -
検出感度
0.014 ng/ml -
検出範囲
0.125 ng/ml - 1 ng/ml -
添加回収試験
97 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s IL-10 Mouse in vitro ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of IL-10 in plasma, serum, tissue extracts and cell culture supernatants.
An IL-10 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an IL-10 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of IL-10 captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Mouse IL-10 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml IL-10 Microplate (12 x 8 well strips) 1 unit IL-10 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
組織特異性
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
配列類似性
Belongs to the IL-10 family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
参照データベース
- Entrez Gene: 16153 Mouse
- SwissProt: P18893 Mouse
- Unigene: 874 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108870
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab108870 は 36 報の論文で使用されています。
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- Yu Y et al. MicroRNA-665-3p exacerbates nonalcoholic fatty liver disease in mice. Bioengineered 13:2927-2942 (2022). PubMed: 35038955
- Hou B et al. Inhibition of poly (ADP-ribose) polymerase 1 in neuron cells might enhance the therapeutic effect of leonurine under cholesterol stimulation. Folia Neuropathol 60:76-91 (2022). PubMed: 35359148
- Zheng S et al. microRNA-129 overexpression in endothelial cell-derived extracellular vesicle influences inflammatory response caused by myocardial ischemia/reperfusion injury. Cell Biol Int 45:1743-1756 (2021). PubMed: 33851774
- Zhang C et al. Arbutin ameliorated ulcerative colitis of mice induced by dextran sodium sulfate (DSS). Bioengineered 12:11707-11715 (2021). PubMed: 34783296