AB254517

Mouse Hemoglobin ELISA Kit (Hba)

Recombinant
SimpleStep
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Mouse Hemoglobin ELISA Kit (Hba) is a single-wash 90-min Simplestep used to quantify Mouse Hemoglobin (Hba) with a sensitivity of 0.06 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Hba-a1, Hba, Hemoglobin subunit alpha, Alpha-globin, Hemoglobin alpha chain

5 Images

ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

ELISA

Supplier Data

ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

sELISA

Supplier Data

Sandwich ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

Example of mouse Hemoglobin standard curve in Sample Diluent NS.

The Hemoglobin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

Interpolated concentrations of native Hemoglobin in mouse liver supernatant samples.

The concentrations of Hemoglobin were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : liver supernatant 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin concentration was determined to be 44.3 ng/mL in liver supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

Interpolated concentrations of native Hemoglobin in mouse kidney based on a 0.5 μg/mL extract load.

The concentrations of Hemoglobin were measured in duplicate and interpolated from the Hemoglobin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Mouse Hemoglobin ELISA Kit (Hba) (AB254517)

Interpolated concentrations of native Hemoglobin in mouse serum and plasma samples.

The concentrations of Hemoglobin were measured in duplicates, interpolated from the Hemoglobin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 4,000, plasma (EDTA) 1 : 4,000, and plasma (heparin) 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hemoglobin concentration was determined to be 27.0 μg/mL in serum, 12.9 μg/mL in plasma (EDTA) and 2.1 μg/mL in plasma (Heparin).

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma, Tissue Extracts

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 0.06 ng/mL

検出範囲

0.125 - 8 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Hemoglobin ELISA Kit (Hba) ab254517 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Hemoglobin (Hba)in cell culture supernatant, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.06 ng/mL.

How the assay works

Mouse Hemoglobin (Hba)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Hemoglobin ELISA Kit (Hba) ab254517 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Save your precious samples

Our 384-well Mouse Hemoglobin ELISA Kit (Hba) only requires a maximum of 12.5µL sample

Hemoglobin (Haemoglobin) subunits combine to form the quaternary structure of total hemoglobin, the oxygen carrying protein in red blood cells. In normal healthy adults, hemoglobin consists of 2 alpha and 2 beta subunits. Hemoglobin alpha subunit can be modified by the glucose on the terminal valine of beta subunits resulting in the Hemoglobin A1C molecule. The resulting A1C molecule is used to measure the average glucose levels in blood over time.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Serum", "range": "101 - 108 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "115 - 123 %", "average": "= 117" }, { "sample": "Heparin Plasma", "range": "100 - 106 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "82 - 96 %", "average": "= 89" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Hemoglobin also known as Hb or HBB is a protein found in red blood cells. It is a tetramer with a molecular mass of approximately 64.5 kDa composed of two alpha and two beta subunits. This protein is predominantly expressed in erythrocytes where it plays an important role in oxygen transport from the lungs to tissues and facilitates the return of carbon dioxide from tissues to the lungs for exhalation.

Biological function summary

Hemoglobin carries oxygen in blood and acts as a buffer in maintaining blood pH. Within the erythrocytes hemoglobin comprises one of the principal components of these cells each molecule binding up to four oxygen molecules. Hemoglobin exists as a complex with heme groups that directly interact with oxygen allowing hemoglobin to perform its respiratory function efficiently.

Pathways

Hemoglobin is important in the circulatory and respiratory pathways. It works in conjunction with proteins like myoglobin which assists with oxygen storage in muscles and cytochrome c which is important in the electron transport chain. Hemoglobin's function aligns with these pathways to maintain proper oxygen and energy levels throughout the body.

Hemoglobin abnormalities lead to conditions such as sickle cell anemia and thalassemia. Sickle cell anemia involves a mutation in the beta-globin gene resulting in deformed red blood cells that hinder oxygen delivery and cause pain due to blockages in blood vessels. Thalassemia results from reduced production of globin chains leading to anemia. These conditions involve aberrations in the hemoglobin or related proteins impacting overall health and requiring clinical management.

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製品プロトコール

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ターゲットの情報

Involved in oxygen transport from the lung to the various peripheral tissues.. Hemopressin. Hemopressin acts as an antagonist peptide of the cannabinoid receptor CNR1. Hemopressin-binding efficiently blocks cannabinoid receptor CNR1 and subsequent signaling.

See full target information Hba

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37162190

2023

Sexual dimorphism in obesity is governed by RELMα regulation of adipose macrophages and eosinophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Li,Rebecca E Ruggiero-Ruff,Yuxin He,Xinru Qiu,Nancy Lainez,Pedro Villa,Adam Godzik,Djurdjica Coss,Meera G Nair

View all publications

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websiteProtocolBooklet

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Mouse Hemoglobin ELISA Kit (Hba)