AB238265

Mouse Granzyme B ELISA Kit

Recombinant
SimpleStep
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(5 Publications)

Mouse Granzyme B ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Mouse Granzyme B with a sensitivity of 24.4 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab326734

別名を表示する

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1

4 Images

ELISA - Mouse Granzyme B ELISA Kit (AB238265)

ELISA

Supplier Data

ELISA - Mouse Granzyme B ELISA Kit (AB238265)

sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Interpolated concentrations of native Granzyme B in mouse treated and untreated spleen supernatant samples. The concentrations of Granzyme B were measured in duplicates, interpolated from the Granzyme B standard curves and corrected for sample dilution. Undiluted samples are as follows : treated mouse spleen supernatant 1 : 67 and untreated mouse spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Granzyme B concentration was determined to be 340.6 ng/mL in treated mouse spleen supernatant and 31.7 ng/ml in untreated mouse spleen supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Representative image of Mouse Granzyme B ELISA Kit (ab238265)

Components shown from left to right :

- Sample diluent NS

- 10X Mouse Granzyme B Capture Antibody

- 10X Mouse Granzyme B Detector Antibody

- Mouse Granzyme B Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent CPR2

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

sELISA

Supplier Data

Sandwich ELISA - Mouse Granzyme B ELISA Kit (AB238265)

Example of mouse Granzyme B standard curve in Sample Diluent NS + Enhancer.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 24.4 pg/mL

検出範囲

188 - 12000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Granzyme B ELISA Kit ab238265 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Granzyme B in cell culture supernatant, plasma, serum. This SimpleStep sensitivity is 24.4 pg/mL.

How the assay works

Mouse Granzyme B SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Granzyme B ELISA Kit ab238265 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Granzyme B antibody [EPR20130-11] - BSA and Azide free (Capture) ab245072
- Anti-Granzyme B antibody [EPR20130-273] - BSA and Azide free (Detector) ab245072

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精度

[ { "reproducibilityType": "Inter", "sample": "Mouse Spleen", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Intra", "sample": "Mouse Spleen", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "94 - 101 %", "average": "= 98" }, { "sample": "Serum", "range": "89 - 103 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "103 - 120 %", "average": "= 109" }, { "sample": "Citrate plasma", "range": "85 - 96 %", "average": "= 90" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238265-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB238265-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB238265-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238265-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238265-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238265-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238265-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB238265-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238265-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme B Detector Antibody", "number":"AB238265-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse Granzyme B Lyophilized recombinant Protein", "number":"AB238265-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse Granzyme B Capture Antibody", "number":"AB238265-CMP07", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.

Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.

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製品プロトコール

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ターゲットの情報

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 3555842, PubMed : 35705808). It cleaves after Asp (PubMed : 35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 35705808).

See full target information Gzmb

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文献 (5)

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websiteProtocolBooklet

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Mouse Granzyme B ELISA Kit