AB278087

Mouse GAS6 ELISA Kit

Recombinant
SimpleStep
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Mouse GAS6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse GAS6 with a sensitivity of 8.4 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

別名を表示する

Growth arrest-specific protein 6, GAS-6, AXL receptor tyrosine kinase ligand, Gas6

2 Images

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB278087)

sELISA

Supplier Data

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB278087)

Example of mouse GAS6 standard curve in Sample Diluent NS.

The GAS6 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse GAS6 ELISA Kit (AB278087)

Interpolated concentrations of native GAS6 in mouse serum, plasma and kidney supernatant samples.

The concentrations of GAS6 were measured in duplicates, interpolated from the GAS6 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 10%, plasma (citrate) 10%, plasma (EDTA) 5%, plasma (heparin) 10% and kidney supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GAS6 concentration was determined to be 45.4 ng/mL in neat serum, 44.3 ng/mL in neat plasma (citrate), 71.8 ng/mL in neat plasma (EDTA), 49.3 ng/mL in neat plasma (heparin) and 2.0 ng/mL in neat kidney supernatant.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture media, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 8.4 pg/mL

検出範囲

78.13 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse GAS6 ELISA kit (ab278087) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse GAS6 protein in mouse serum, plasma and cell culture supernatant.. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GAS6 with 8.4 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Gas6 (Growth arrest-specific protein 6), also known as AXL receptor tyrosine kinase ligand, is a secreted ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. Gas6 signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "100 - 118 %", "average": "= 106" }, { "sample": "Serum", "range": "92 - 118 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "103 - 116 %", "average": "= 108" }, { "sample": "Cell culture media", "range": "87 - 90 %", "average": "= 89" }, { "sample": "Heparin Plasma", "range": "67 - 99 %", "average": "= 83" }, { "sample": "Citrate plasma", "range": "94 - 114 %", "average": "= 105" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein Gas6 also known as growth arrest-specific 6 weighs approximately 75 kDa and functions mechanically as a ligand for the TAM (Tyro3 Axl and Mer) receptor tyrosine kinases. Gas6 protein facilitates cell signaling by binding especially to AXL receptor initiating various downstream cellular responses. It is widely expressed in different tissues including the nervous system cardiovascular system and kidneys. Additionally its expression increases in response to cellular stress and inflammatory stimuli.

Biological function summary

Gas6 influences several cellular processes such as cell survival proliferation and migration. Gas6 exerts its effects in a complex with AXL receptor which is part of a larger network involving other TAM receptors. By activating AXL Gas6 modulates immune responses inflammation and tissue repair. The protein also plays a significant role in phagocytosis helping remove apoptotic cells thereby maintaining tissue homeostasis.

Pathways

The Gas6 protein is significantly engaged in the PI3K/AKT signaling pathway important for promoting cell survival and inhibiting apoptosis. Additionally Gas6 interacts with the NF-kB pathway which regulates inflammation and immune responses. Both pathways highlight the synergy between Gas6 and AXL emphasizing their contribution to cellular integrity and response to injury.

Gas6's dysregulation associates with cancer and atherosclerosis. In oncogenesis altered Gas6-AXL signaling promotes tumor progression and resistance to therapy implicating it in diverse cancers. Meanwhile in cardiovascular diseases elevated Gas6 levels correlate with atherosclerosis interacting with inflammatory proteins such as interleukins. Understanding Gas6's role in these conditions offers potential for therapeutic targeting and biomarker development.

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製品プロトコール

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ターゲットの情報

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

See full target information Gas6

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 19:e1011139 PubMed37289655

2023

Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Hitoshi Tsugawa,Takuto Ohki,Shogo Tsubaki,Rika Tanaka,Juntaro Matsuzaki,Hidekazu Suzuki,Katsuto Hozumi

View all publications

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websiteProtocolBooklet

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Mouse GAS6 ELISA Kit