Mouse Fibrinogen ELISA Kit (ab108844)
Key features and details
- Sensitivity: 1.1 µg/ml
- Range: 0.625 µg/ml - 20 µg/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse
製品の概要
-
製品名
Mouse Fibrinogen ELISA Kit
Fibrinogen キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10% -
サンプルの種類
Plasma -
アッセイタイプ
Competitive -
検出感度
= 1.1 µg/ml -
検出範囲
0.625 µg/ml - 20 µg/ml -
添加回収試験
93 %
-
全工程の試験時間
3h 0m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse -
製品の概要
Abcam’s Fibrinogen Mouse in vitro competitive ELISA (enzyme-linked immunosorbent assay) kit is designed for the quantitative measurement of mouse fibrinogen in plasma.
A Fibrinogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a biotinylated Fibrinogen is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Fibrinogen captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
-
アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 2X Biotinylated Mouse Fibrinogen (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Fibrinogen Microplate (12 x 8 well strips) 1 unit Fibrinogen Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
-
機能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
組織特異性
Plasma. -
関連疾患
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻訳後修飾
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- FGA
- FGB
- FGG
see all -
参照データベース
- Entrez Gene: 14161 Mouse
- Entrez Gene: 110135 Mouse
- Entrez Gene: 99571 Mouse
- SwissProt: Q8K0E8 Mouse
- SwissProt: Q8VCM7 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108844の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
-
Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
-
Competitive ELISA - Fibrinogen Mouse ELISA Kit (ab108844)Fibrinogen measured in biological fluids showing quantity (ug) per mL of tested sample. Samples diluted 1-500 fold.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab108844 は 4 報の論文で使用されています。
- Yuan C et al. Novel 1-hydroxy phenothiazinium-based derivative protects against bacterial sepsis by inhibiting AAK1-mediated LPS internalization and caspase-11 signaling. Cell Death Dis 13:722 (2022). PubMed: 35982051
- Hasselwander S et al. B lymphocyte-deficiency in mice promotes venous thrombosis. Heliyon 8:e11740 (2022). PubMed: 36439760
- Makhloufi C et al. Assessment of Thrombotic and Bleeding Tendency in Two Mouse Models of Chronic Kidney Disease: Adenine-Diet and 5/6th Nephrectomy. TH Open 4:e66-e76 (2020). PubMed: 32309772
- de la Rosa X et al. Mannose-binding lectin promotes local microvascular thrombosis after transient brain ischemia in mice. Stroke 45:1453-9 (2014). PubMed: 24676774