Mouse FAP ELISA Kit (ab289903)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.052 pg/ml
- Range: 34.375 pg/ml - 2200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse FAP ELISA Kit
Fibroblast activation protein, alpha キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Serum 8 2.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Serum 3 5.9% -
サンプルの種類
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
>= 6.052 pg/ml -
検出範囲
34.375 pg/ml - 2200 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 81 74% - 90% Serum 106 100% - 110% Hep Plasma 104 100% - 105% EDTA Plasma 104 101% - 106% Cit plasma 107 101% - 118% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Cow, Human, Monkey -
製品の概要
Mouse FAP ELISA Kit (ab289903) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse FAP in cell culture supernatant, plasma and serum samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse FAP with 6.052 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 10X Mouse FAP Capture Antibody 1 x 600µl 1 x 600µl 10X Mouse FAP Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent CPR2 1 x 6ml 1 x 6ml Mouse FAP Lyophilized Recombinant Protein 2 vials 2 vials Plate Seal 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究分野
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機能
In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers. -
組織特異性
Fibroblast specific. -
配列類似性
Belongs to the peptidase S9B family. -
翻訳後修飾
N-glycosylated.
The N-terminus may be blocked. -
細胞内局在
Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. - Information by UniProt
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別名
- 170 kDa melanoma membrane bound gelatinase
- 170 kDa melanoma membrane-bound gelatinase
- DPPIV
see all -
参照データベース
- Entrez Gene: 14089 Mouse
- SwissProt: P97321 Mouse
- Unigene: 41816 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289903の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Example of mouse FAP standard curve in Sample Diluent NS.
Example of mouse FAP standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of mouse FAP in serum, plasma (EDTA), plasma (citrate), and plasma (heparin).Interpolated concentration of native FAP was measured in duplicate at different sample concentrations. Undiluted samples are 1:2,000 serum, plasma (EDTA), plasma (citrate), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Interpolated concentrations of mouse FAP in liver tissue culture supernatant.Interpolated concentration of native FAP was measured in duplicate at different sample concentrations. Undiluted samples are 12.5% liver tissue culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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Mouse FAP ELISA Kit (ab289903) -
Mouse FAP ELISA Kit (ab289903)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289903 は論文での使用が確認できていません。