AB204527

Mouse CD31 ELISA Kit (PECAM1)

Recombinant
SimpleStep
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(4 Publications)

Mouse CD31 ELISA Kit (PECAM1) is a single-wash 90-min SimpleStep ELISA used to quantify Mouse CD31 (PECAM1) with a sensitivity of 16.4 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab327869

別名を表示する

CD31, Pecam, Pecam-1, Pecam1, Platelet endothelial cell adhesion molecule, PECAM-1

3 Images

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

sELISA

Supplier Data

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

Titration of mouse serum, citrate plasma, heparin plasma, and EDTA plasma within the working range of the assay.

The 1X dilution was 6.25% for the mouse serum and plasma citrate, 3.13% for plasma EDTA, and 100% for plasma heparin. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

Titration of mouse unstimulated (-) liver supernatant.

A blank cell culture media control was also analyzed, and was negative. The 1X dilution was 50%. Interpolated data values (mean +/- SD, n = 2) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse CD31 ELISA Kit (PECAM1) (AB204527)

Example of CD31 standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 16.4 pg/mL

検出範囲

125 - 8000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Microplate (12 x 8 well strips)

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Reactivity data

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製品の詳細

Mouse CD31 ELISA Kit (PECAM1) (ab204527) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD31 (PECAM1) protein in cell culture supernatant, serum, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CD31 (PECAM1) with 16.4 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

CD31 is a cell adhesion molecule, and a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and particular T-cell subsets. It is a major constituent of the endothelial cell intercellular junction, and is implicated in transendothelial migration of leukocytes, angiogenesis, and integrin activation. CD31 also prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes.

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.25" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 6, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.52" } ]

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回収率

[ { "sample": "Serum", "range": "77 - 87 %", "average": "= 84" }, { "sample": "EDTA Plasma", "range": "84 - 86 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "77 - 90 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "89 - 94 %", "average": "= 92" }, { "sample": "Cell culture media", "range": "74 - 92 %", "average": "= 85" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed : 19048083).

See full target information Pecam1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70176 PubMed39835780

2025

Annexin A8 deficiency delays atherosclerosis progression.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Gutiérrez-Muñoz,Rafael Blázquez-Serra,Irene San Sebastian-Jaraba,Sandra Sanz-Andrea,Maria J Fernández-Gómez,Gonzalo Nuñez-Moreno,Pablo Mínguez,Joan Carles Escolá-Gil,Paula Nogales,Veronique Ollivier,Jose L Martín-Ventura,Benoit Ho-Tin-Noe,Ursula Rescher,Nerea Méndez-Barbero,Luis M Blanco-Colio

Heliyon 10:e37231 PubMed39296039

2024

PMMA-induced biofilm promotes Schwann cells migration and proliferation mediated by EGF/Tnc/FN1 to improve sciatic nerve defect.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,YuXuan Hu,Yuan Xue,Kai Wang,Dong Mao,Xiao-Yun Pan,YongJun Rui

Integrative cancer therapies 22:15347354231198195 PubMed37694878

2023

Effect of Moxibustion Combined With Cisplatin on Tumor Microenvironment Hypoxia and Vascular Normalization in Lewis Lung Cancer Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Mao,Xiaobo Wu,Chaofeng Wang,Huijuan Mao,Jianzi Wei

Investigative ophthalmology & visual science 64:13 PubMed37279396

2023

MicroRNA 9 Is a Regulator of Endothelial to Mesenchymal Transition in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Wang,Biao Feng,Subrata Chakrabarti

View all publications

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websiteProtocolBooklet

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Mouse CD31 ELISA Kit (PECAM1)