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AB324138

Mouse C-Peptide ELISA Kit

Mouse C-Peptide ELISA Kit

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Mouse C-Peptide ELISA Kit is a Sandwich ELISA for the measurement of Mouse C-Peptide in Mouse in Biofluids, Cell Culture Media samples.

別名を表示する

Ins-1, Ins1, Insulin-1

2 Images
Sandwich ELISA - Mouse C-Peptide ELISA Kit (AB324138)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse C-Peptide ELISA Kit (AB324138)

Example of mouse C-Peptide standard curve in 1X Sample Diluent PT. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse C-Peptide ELISA Kit (AB324138)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse C-Peptide ELISA Kit (AB324138)

Interpolated concentration of native C-Peptide was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 50%, plasma (citrate) 25%, and plasma (EDTA) 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Sample Diluent PT.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, EDTA Plasma, Cell culture media

交差種

Mouse

アッセイタイプ

Sandwich

検出感度

= 1.16 pg/mL

検出範囲

3.516 - 225 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Mouse C-Peptide SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of C-Peptide protein in Serum, Cit Plasma, EDTA Plasma, Cell culture media. Quantitate Mouse C-Peptide with 1.16 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  • Singlewash protocol reduces assay time to 90 minutes or less.
  • High sensitivity, specificity and reproducibility from superior antibodies.
  • Fully validated in biological samples.
  • 96wells plate breakable into 12 x 8 wells strips, available in 10pack (10 x 96well plates).
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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.9" } ]
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回収率

[ { "sample": "Serum", "range": "102 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "91 - 95 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "91 - 97 %", "average": "= 95" }, { "sample": "Cell culture media", "range": "88 - 90 %", "average": "= 89" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information Ins1
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websiteProtocolBooklet
en
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