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AB263882

Mouse AST ELISA Kit (Aspartate Aminotransferase)

Mouse AST ELISA Kit (Aspartate Aminotransferase)

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(51 Publications)

Mouse AST ELISA Kit (Aspartate Aminotransferase) is a single-wash 90-min SimpleStep ELISA used to quantify Mouse AST (Aspartate Aminotransferase) with a sensitivity of 39 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab325368

別名を表示する

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, Got1

8 Images
ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • ELISA

Supplier Data

ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentrations of native AST in mouse serum and plasma samples.

The concentrations of AST were measured in duplicates, interpolated from the AST standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 40, plasma (EDTA) 1 : 40 and plasma (heparin) 1 : 40. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 271 ng/mL in serum, 307 ng/mL in plasma (citrate), 197 ng/mL in plasma (EDTA), and 190 ng/mL in plasma (heparin).

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in Sample Diluent NS.

The AST standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentrations of native AST in mouse liver extract and NIH3T3 cell extract samples based on a 3,125 ng/mL and 12,500 ng/mL extract load, respectively.

The concentrations of AST were measured in duplicate, interpolated from the AST standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AST concentration was determined to be 4,352 pg/mL in mouse liver extract and 6,385 pg/mL in NIH3T3 cell extract.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1,560 ng/mL mouse liver extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Example of mouse AST standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)
  • sELISA

Lab

Sandwich ELISA - Mouse AST ELISA Kit (Aspartate Aminotransferase) (AB263882)

Interpolated concentration of native AST was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 40 mouse serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Lysate, Heparin Plasma, Citrate plasma, Serum, Cell Lysate, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative
検出感度

= 39 pg/mL

検出範囲

125 - 8000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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組合せ製品

Assay Kits

AB325368

Mouse Aspartate Aminotransferase (AST) ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA

Sandwich ELISA - Mouse Aspartate Aminotransferase (AST) ELISA Kit, Chemiluminescent (AB325368)

  • 0
  • 0
Assay Kits

AB282882

Mouse ALT ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse ALT ELISA Kit (AB282882)

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Assay Kits

AB179887

Human Albumin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Albumin ELISA Kit (AB179887)

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Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB105134

Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric)

Functional Studies - Alanine Transaminase Activity Assay Kit (Colorimetric/Fluorometric) (AB105134)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 is a rapid single-wash 90-min ELISA kit to measure tissue lysate, cell lysate, serum and plasma. This SimpleStep ELISA® sensitivity is 39 pg/mL.

How the assay works
SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of mouse AST. The standard protein in this kit is full length mouse AST. Native signal was detected in serum, plasma (citrate), plasma (EDTA), plasma (heparin), cell extract, and tissue extract sample types. Cell culture supernatant, urine, saliva, milk, and CSF samples have not been tested with this kit.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and assayed for cross reactivity. No cross-reactivity was observed.
Recombinant Human IL-1 beta, Mouse IL-1 alpha, and Mouse IL-1 receptor1 were prepared at 5 ng/mL and tested for interference. No interference with was observed.

Mouse AST ELISA Kit (Aspartate Aminotransferase) ab263882 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Mouse AST ELISA Kit ab263882
Mouse AST ELISA Kit ab263882 has been used to measure AST plasma to study mitochondrial homeostatis in liver disease(1) and rencofilstat as a treatment for HCV-induced hepatocellular carcinoma(2).

References:
(1)Ai-Lei Xu et al. 2024, PMID: 37902450,
(2)Philippe Gallay et al. 2023, PMID: 37896876

Save your precious samples
Our 384-well Mouse AST ELISA Kit (Aspartate Aminotransferase) only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Mouse/Rat Aspartate Aminotransferase Antibody Pair - BSA and Azide free ab267702
- Anti-Aspartate Aminotransferase antibody [EPR20781-22] - BSA and Azide free (Capture) ab259695
- Anti-Aspartate Aminotransferase antibody [EPR22206-177] - BSA and Azide free (Detector) ab259696

Related and recommended products
Mouse AST ELISA Kit ab263882 is commonly used by researchers to measure liver function.

Other ELISA kits to measure liver function include:
- Mouse ALT ELISA kit ab234578
- Mouse Albumin ELISA kit ab282297
- Mouse Alkaline Phosphatase ELISA kit ab285274

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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回収率

[ { "sample": "Serum", "range": "90 - 105 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "87 - 104 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "94 - 107 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "110 - 118 %", "average": "= 115" }, { "sample": "Cell Lysate", "range": "99 - 102 %", "average": "= 101" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
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製品プロトコール

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ターゲットの情報

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
See full target information Got1
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文献 (51)

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