AB326384

Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
Recombinant
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Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Mouse alpha 1 acid Glycoprotein with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 41.15 - 90000 pg/ml with a sensitivity of 12.36pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

別名を表示する

Agp1, Orm-1, Orm1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

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Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

sELISA

Lab

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

Dynamic range comparison between the Chemiluminescent Mouse alpha 1 acid Glycoprotein SimpleStep ELISA, colorimetric Mouse alpha 1 acid Glycoprotein SimpleStep ELISA (ab264605), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

sELISA

Lab

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

Standard curve comparison between the Chemiluminescent Mouse alpha 1 acid Glycoprotein SimpleStep ELISA and colorimetric Mouse alpha 1 acid Glycoprotein SimpleStep ELISA (ab264605) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

Interpolated concentrations of native alpha 1 acid Glycoprotein in male mouse urine samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : male mouse urine 1 : 256. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 1.94 µg/mL in male mouse urine.

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse serum and plasma samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 2000 plasma (citrate) 1 : 8000 plasma (heparin) 1 : 4000 and plasma (EDTA) 1 : 2000. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 16.84 µg/mL in serum 47.40 µg/mL in plasma (citrate) 24.08 µg/ml in plasma (heparin) and 17.47 µg/ml in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (AB326384)

Interpolated concentrations of native alpha 1 acid Glycoprotein in mouse liver tissue culture supernatant samples.

The concentrations of alpha 1 acid Glycoprotein were measured in duplicates interpolated from the alpha 1 acid Glycoprotein standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse liver supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean alpha 1 acid Glycoprotein concentration was determined to be 10.66 ng/mL in liver supernatant.

Key facts

検出方法

Luminescent

サンプルタイプ

Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 12.36 pg/mL

検出範囲

41.15 - 90000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (ab326384) is a rapid single-wash sandwich 55-min ELISA to measure Mouse alpha 1 acid Glycoprotein in Cell culture supernatant, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 12.36 pg/mL.

How the assay works

Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent (ab326384) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse alpha 1 acid Glycoprotein (ab326384) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR22456-88] - BSA and Azide free (Capture) ab270313
- Anti-Alpha 1 Acid Glycoprotein/AGP antibody [EPR22456-65] - BSA and Azide free (Detector) ab270314

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.2" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.4" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "98 - 112 %", "average": "= 103" }, { "sample": "Serum", "range": "99 - 108 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "108 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "100 - 116 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "94 - 112 %", "average": "= 103" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Functions as a transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain (By similarity). Appears to function in modulating the activity of the immune system during the acute-phase reaction.

See full target information Orm1

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websiteProtocolBooklet

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Abcam product promise

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Mouse alpha 1 acid Glycoprotein ELISA Kit, Chemiluminescent