Mouse Albumin ELISA Kit (ab108791)
Key features and details
- Sensitivity: 1.4 µg/ml
- Range: 1.563 µg/ml - 100 µg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Mouse
製品の概要
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製品名
Mouse Albumin ELISA Kit
Albumin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 9.6% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Competitive -
検出感度
= 1.4 µg/ml -
検出範囲
1.563 µg/ml - 100 µg/ml -
添加回収試験
= 96 %
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全工程の試験時間
2h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Mouse
交差が予測される動物種: Rat
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製品の概要
Mouse Albumin ELISA kit (ab108791) is a competitive ELISA designed for the quantitative measurement of mouse Albumin levels in plasma and serum.
An Albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Albumin is added and then followed by washing with PBS or TBS buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Albumin captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Competitive ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 2X Biotinylated Mouse Albumin (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Albumin Microplate (12 x 8 wells) 1 test Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
組織特異性
Plasma. -
関連疾患
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
細胞内局在
Secreted. - Information by UniProt
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別名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
参照データベース
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- SwissProt: P07724 Mouse
- SwissProt: P02770 Rat
- Unigene: 16773 Mouse
- Unigene: 202968 Rat
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Competitive ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Competitive ELISA
Use at an assay dependent concentration. |
画像
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Mouse Albumin ELISA Kit example standard curve
Mouse Albumin ELISA Kit example standard curve – data provided for demonstration purposes only.
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Competitive ELISA - Albumin Mouse ELISA Kit (ab108791)Albumin measured in mouse tissue lysates (expressed as per mg of extracted protein), with background signal subtracted (duplicates; +/- SD). -
Competitive ELISA - Albumin Mouse ELISA Kit (ab108791)Albumin measured in biological fluids, background signal subtracted (duplicates +/- SD).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (16)
ab108791 は 16 報の論文で使用されています。
- Chen J et al. Diabetic kidney disease-predisposing proinflammatory and profibrotic genes identified by weighted gene co-expression network analysis (WGCNA). J Cell Biochem 123:481-492 (2022). PubMed: 34908186
- Tsujinaka H et al. Sustained treatment of retinal vascular diseases with self-aggregating sunitinib microparticles. Nat Commun 11:694 (2020). PubMed: 32019921
- Wen S et al. Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis. Diabetes Metab Syndr Obes 13:313-323 (2020). PubMed: 32104028
- Lorenc VE et al. Hepatocyte growth factor is upregulated in ischemic retina and contributes to retinal vascular leakage and neovascularization. FASEB Bioadv 2:219-233 (2020). PubMed: 32259049
- Hoffman JB et al. Prebiotic inulin consumption reduces dioxin-like PCB 126-mediated hepatotoxicity and gut dysbiosis in hyperlipidemic Ldlr deficient mice. Environ Pollut 261:114183 (2020). PubMed: 32105967