AB226900

Mouse Adiponectin ELISA Kit

SimpleStep
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(3 Publications)

Mouse Adiponectin ELISA Kit is a single-wash 90-min SimpleStep ELISA used to quantify Mouse Adiponectin with a sensitivity of 26 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- For higher sensitivity and a broader dynamic range, check our chemiluminescent SimpleStep Ignite™ ab325907

別名を表示する

Acdc, Acrp30, Apm1, Adipoq, Adiponectin, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte-specific protein AdipoQ, ACRP30

5 Images

ELISA - Mouse Adiponectin ELISA Kit (AB226900)

ELISA

Supplier Data

ELISA - Mouse Adiponectin ELISA Kit (AB226900)

sELISA

Supplier Data

Sandwich ELISA - Mouse Adiponectin ELISA Kit (AB226900)

Interpolated concentrations of native Adiponectin in Mouse cell culture supernatant samples.

The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : lung supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 386 pg/mL in lung supernatant.

sELISA

Supplier Data

Sandwich ELISA - Mouse Adiponectin ELISA Kit (AB226900)

Interpolated concentrations of native Adiponectin in Mouse serum, and plasma samples.

The concentrations of Adiponectin were measured in duplicates, interpolated from the Adiponectin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 666, plasma (EDTA) 1 : 666, plasma (heparin) 1 : 666, and plasma (citrate) 1 : 666. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Adiponectin concentration was determined to be 2212 ng/mL in serum, 2168 ng/mL in plasma (EDTA), 2129 ng/mL in plasma (heparin) and 1845 ng/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Mouse Adiponectin ELISA Kit (AB226900)

Example of Mouse Adiponectin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Mouse Adiponectin ELISA Kit (AB226900)

Mouse Adiponectin Competitor standard curve comparison.

Standard curve comparison between Mouse Adiponectin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

検出方法

Colorimetric

サンプルタイプ

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Mouse

アッセイタイプ

Sandwich

Quantitative

検出感度

= 26 pg/mL

検出範囲

78.1 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Mouse Adiponectin ELISA Kit ab226900 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse Adiponectin in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 26 pg/mL.

How the assay works

Mouse Adiponectin SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse Adiponectin ELISA Kit ab226900 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Adiponectin is a 30kDa monomer that combines to form trimers (low molecular weight), hexamers (middle molecular weight), and other higher molecular weight multimers. Adiponectin is an important adipokine involved in fat metabolism and insulin sensitivity. Insulin stimulates the secretion of adiponectin. Decreased levels of adiponectin are associated with insulin resistance, whereas high levels of adiponectin are associated with reduced risk of type-2 diabetes.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]

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回収率

[ { "sample": "Serum", "range": "106 - 109 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "93 - 105 %", "average": "= 99" }, { "sample": "Cell culture media", "range": "99 - 106 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "102 - 116 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "109 - 114 %", "average": "= 112" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Adiponectin also known as ADIPOQ or adipocyte complement-related protein of 30 kDa (Acrp30) is a 30 kDa protein that plays a significant mechanical role in energy homeostasis. Adiponectin is secreted predominantly by adipose tissue and circulates in the blood. This protein forms various multimeric structures including low-molecular-weight medium-molecular-weight and high-molecular-weight forms each with distinct biological functions. Its expression in adipose tissue links it closely to metabolic processes and functions related to fat storage and energy balance.

Biological function summary

Adiponectin influences glucose regulation and fatty acid oxidation. It acts as a hormone with several metabolic roles including anti-diabetic anti-atherogenic and anti-inflammatory properties. Adiponectin participates in forming a complex with other proteins such as AdipoR1 and AdipoR2 which are receptors facilitating signal transduction. The interaction of adiponectin with its receptors leads to the induction of several lipid and glucose metabolism pathways important for maintaining cellular energy balance.

Pathways

Many regulatory cascades are influenced by adiponectin. This protein is integral to the AMPK signaling pathway and the PPAR signaling pathway. In the AMPK pathway adiponectin enhances insulin sensitivity and stimulates oxidation of fatty acids in muscle tissue. Through the PPAR pathway it influences lipid metabolism and storage. Adiponectin interacts with key proteins such as leptin and resistin coordinating various metabolic pathways to balance energy and glucose levels making it a critical factor in metabolic regulation.

Reduced levels of adiponectin associate with conditions like type 2 diabetes and cardiovascular disease. Lower circulating adiponectin concentrations correlate with obesity leading to increased insulin resistance and higher risk of type 2 diabetes. In cardiovascular disease this protein's role links to its anti-inflammatory properties and ability to decrease the proliferation of vascular smooth muscle cells. Adiponectin also interacts with proteins like cytokines that play a role in these conditions affecting the body’s inflammatory responses and metabolic processes highlighting its importance in health and disease management.

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製品プロトコール

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ターゲットの情報

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.

See full target information Adipoq

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23039 PubMed37392374

2023

CCR2 inhibitor strengthens the adiponectin effects against myocardial injury after infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zheng,Wenqing Gao,Bingcai Qi,Ruiying Zhang,Meng Ning,Xiaomin Hu,Tong Li

Nutrients 14: PubMed36501204

2022

Anti-Obesity Effects of Multi-Strain Probiotics in Mice with High-Carbohydrate Diet-Induced Obesity and the Underlying Molecular Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Hye Rim Kim,Eunsol Seo,Seyeon Oh,MinYeong Seo,Kyunghee Byun,Byung-Yong Kim

Communications biology 2:389 PubMed31667363

2019

Adipocyte browning and resistance to obesity in mice is induced by expression of ATF3.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Feng Cheng,Hui-Chen Ku,Jing-Jy Cheng,Shi-Wei Chao,Hsiao-Fen Li,Pei-Fang Lai,Che-Chang Chang,Ming-Jaw Don,Hsi-Hsien Chen,Heng Lin

View all publications

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websiteProtocolBooklet

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Mouse Adiponectin ELISA Kit