AB317547

Monkey IL-11 ELISA Kit

SimpleStep
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Monkey IL-11 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-11 with a sensitivity of 26.73 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

別名を表示する

Interleukin-11, IL-11, IL11, IL 11, Interleukin 11

2 Images

Sandwich ELISA - Monkey IL-11 ELISA Kit (AB317547)

sELISA

Supplier Data

Sandwich ELISA - Monkey IL-11 ELISA Kit (AB317547)

Example of monkey IL-11 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey IL-11 ELISA Kit (AB317547)

Example of monkey IL-11 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

EDTA Plasma, Cell culture supernatant, Heparin Plasma

交差種

Monkey

アッセイタイプ

Sandwich

検出感度

= 26.73 pg/mL

検出範囲

156.25 - 10000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Monkey IL-11 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-11 protein in EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Monkey IL-11 with 26.73 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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回収率

[ { "sample": "EDTA Plasma", "range": "81 - 103 %", "average": "= 93" }, { "sample": "Cell culture supernatant", "range": "108 - 115 %", "average": "= 112" }, { "sample": "Heparin Plasma", "range": "94 - 106 %", "average": "= 99" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab317547-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB317547-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB317547-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317547-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB317547-CMP07" }, { "size":"20 x 1 Vial", "name":"Monkey IL-11 Lyophilized Recombinant Protein", "number":"AB317547-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB317547-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317547-CMP05" }, { "size":"10 x 600 µL", "name":"10X Monkey IL-11 Capture Antibody", "number":"AB317547-CMP01" }, { "size":"10 x 600 µL", "name":"10X Monkey IL-11 Detector Antibody", "number":"AB317547-CMP02" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317547-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab317547-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB317547-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB317547-CMP08" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317547-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB317547-CMP07" }, { "size":"2 x 1 Vial", "name":"Monkey IL-11 Lyophilized Recombinant Protein", "number":"AB317547-CMP03" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317547-CMP05" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB317547-CMP04" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-11 Capture Antibody", "number":"AB317547-CMP01" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB317547-CMP11" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-11 Detector Antibody", "number":"AB317547-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab317547-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB317547-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB317547-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB317547-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB317547-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB317547-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB317547-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Monkey IL-11 Lyophilized Recombinant Protein", "number":"AB317547-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-11 Capture Antibody", "number":"AB317547-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Monkey IL-11 Detector Antibody", "number":"AB317547-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB317547-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'.

See full target information IL11

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websiteProtocolBooklet

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Abcam product promise

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Monkey IL-11 ELISA Kit