AB317549

Monkey Eotaxin ELISA Kit

SimpleStep
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Monkey Eotaxin ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey Eotaxin with a sensitivity of 12.913 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput

別名を表示する

SCYA11, CCL11, Eotaxin, C-C motif chemokine 11, Small-inducible cytokine A11

2 Images

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

sELISA

Supplier Data

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

Example of monkey Eotaxin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Monkey Eotaxin ELISA Kit (AB317549)

Example of monkey Eotaxin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Citrate plasma

交差種

Monkey

アッセイタイプ

Sandwich

Quantitative

検出感度

= 12.913 pg/mL

検出範囲

39.063 - 2500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Monkey Eotaxin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin protein in Serum, EDTA Plasma, Cit Plasma. Quantitate Monkey Eotaxin with 12.913 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" } ]

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回収率

[ { "sample": "Serum", "range": "89 - 101 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "97 - 107 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "77 - 82 %", "average": "= 79" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Eotaxin also known as CCL11 or eotaxin-1 is a chemokine belonging to the CC chemokine family. This protein has a molecular mass of approximately 8.4 kDa. Eotaxin is mainly expressed in epithelial cells fibroblasts and smooth muscle cells. High levels of expression occur during inflammatory processes where it acts as a chemoattractant guiding eosinophils to sites of inflammation.

Biological function summary

Eotaxin plays an important role in immune response regulation particularly in allergic reactions. It functions by binding to CCR3 a receptor found on eosinophils therefore facilitating their migration. This process includes involvement in the formation of protein complexes that modulate cell signaling. Eotaxin's primary biological role lies in promoting the accumulation of eosinophils which are important for the immune defense against parasitic infections.

Pathways

Eotaxin participates in the MAPK/ERK signaling pathway which influences cell migration and proliferation. Additionally it operates within the NF-kB pathway often regulating inflammation and immune response. In these pathways eotaxin interacts with other proteins like CCR3 and IL-5 contributing to a coordinated response in immune signaling.

Eotaxin is closely linked to allergic asthma and atopic dermatitis. In both these conditions eotaxin-mediated eosinophil recruitment and activation worsen inflammation. Research shows significant eotaxin expression in asthmatic airway tissues. It also connects with proteins like IL-5 which amplify eosinophilic activity leading to exacerbated allergic responses.

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製品プロトコール

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ターゲットの情報

Chemokine that plays a central role in both allergic and non-allergic inflammatory reactions by inducing the migration of different leukocyte types including eosinophils, basophils, macrophages and dendritic cells. Exerts its effects primarily by binding to the CCR3 receptor. Induces also chemotaxis of endothelial cells and promotes angiogenesis by activating the PI3K/Akt pathway.

See full target information CCL11

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websiteProtocolBooklet

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Monkey Eotaxin ELISA Kit