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AB327316

Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent

Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent

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Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Monkey CRP (C-Reactive Protein) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 9.14 - 20000 pg/ml with a sensitivity of 1.65 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
4 Images
Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)

Interpolated concentrations of CRP in rhesus macaque cerebrospinal fluid.

Interpolated concentration of native CRP was measured in duplicate at different sample concentrations. Undiluted samples are 3.13% rhesus macaque cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)
  • sELISA

Supplier Data

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)

Interpolated concentrations of native CRP in monkey serum and plasma samples.

The concentrations of CRP were measured in duplicates interpolated from the CRP standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 500 plasma (citrate) 1 : 500 plasma (EDTA) 1 : 2000 and plasma (heparin) 1 : 500. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean CRP concentration was determined to be 824.7 ng/mL in serum 743.0 ng/mL in plasma (citrate) and 2689.5 ng/mL in plasma (EDTA) and 826.5 ng/mL in plasma (heparin).

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)
  • sELISA

Lab

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)

Standard curve comparison between the Chemiluminescent Monkey CRP SimpleStep ELISA and colorimetric Monkey CRP SimpleStep ELISA (ab260062) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)
  • sELISA

Lab

Sandwich ELISA - Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (AB327316)

Dynamic range comparison between the Chemiluminescent Monkey CRP SimpleStep ELISA, colorimetric Monkey CRP SimpleStep ELISA (ab260062), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

検出方法

Luminescent

サンプルタイプ

Cerebral Spinal Fluid, Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma

交差種

Monkey

アッセイタイプ

Sandwich

Quantitative
検出感度

= 1.65 pg/mL

検出範囲

9.14 - 20000 pg/mL

アッセイ時間

55m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (ab327316) is a rapid single-wash sandwich 55-min ELISA to measure Monkey CRP (C-Reactive Protein) in Cerebral Spinal Fluid, Serum, EDTA Plasma, Cell culture media, Heparin Plasma, Citrate plasma with a sensitivity of 1.65 pg/mL.

How the assay works

Monkey CRP (C-Reactive Protein) ELISA Kit, Chemiluminescent (ab327316) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Monkey CRP (C-Reactive Protein) (ab327316) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-CRP antibody [EPR20063-79] - BSA and Aside free (Capture) ab259539
- Anti-CRP antibody [EPR20063-85] - BSA and Aside free (Detector) ab259540
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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" } ]
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回収率

[ { "sample": "Cerebral Spinal Fluid", "range": "99 - 110 %", "average": "= 104" }, { "sample": "Serum", "range": "110 - 119 %", "average": "= 115" }, { "sample": "EDTA Plasma", "range": "111 - 117 %", "average": "= 115" }, { "sample": "Cell culture media", "range": "97 - 103 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "112 - 115 %", "average": "= 113" }, { "sample": "Citrate plasma", "range": "113 - 117 %", "average": "= 115" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

See full target information CRP
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websiteProtocolBooklet
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Abcam product promise

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