AB126450

Met (pY1234/pY1235) ELISA Kit

Name: Met (pY1234/pY1235) ELISA Kit (ab126450) | Abcam
Brand: Abcam Limited
SKU: AB126450
Price: 145000 JPY
Availability: InStock

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Met (pY1234/pY1235) ELISA Kit is a ELISA for the measurement of Met (pY1234/pY1235) in Human, Mouse in Cell/Tissue Extracts samples.

別名を表示する

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET

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Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

A431 cells were treated or untreated with 50 ng/ml recombinant Human HGF for 5 min. Cell lysates were analyzed using ab126450.

sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.

sELISA

Supplier Data

Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.

Supplier Data

Western blot - Met (pY1234/pY1235) ELISA Kit (AB126450)

H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.

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Key facts

検出方法

Colorimetric

サンプルタイプ

Cell Lysate

交差種

Mouse, Human

Semi-Quantitative

アッセイ時間

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

ab126450 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human and Mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-Met (Tyr1234/1235). An anti-pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Met (Tyr1234/1235) antibody is used to detect phosphorylated Met (Tyr1234/1235). After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab126450-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB126450-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB126450-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Met Microplate (12 strips x 8 wells) coated with anti-pan Met antibody", "number":"AB126450-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB126450-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control: lyophilized powder from H1993 cell lysate", "number":"AB126450-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB126450-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB126450-CMP06", "productcode":"" }, { "size":"1 x 25 µL", "name":"1000X HRP-conjugated anti-rabbit IgG", "number":"AB126450-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Detection Antibody Met (Y1234/1235)", "number":"AB126450-CMP09", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (By similarity). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Also promotes differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).. (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.

See full target information MET phospho Y1235

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Abcam product promise

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Met (pY1234/pY1235) ELISA Kit