AB255719

Human YKL-40 ELISA Kit

Recombinant
SimpleStep
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(7 Publications)

Human YKL-40 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human YKL-40 with a sensitivity of 3.9 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

別名を表示する

Chitinase-3-like protein 1, 39 kDa synovial protein, Cartilage glycoprotein 39, YKL-40, CGP-39, GP-39, hCGP-39, CHI3L1

6 Images

ELISA - Human YKL-40 ELISA Kit (AB255719)

ELISA

Supplier Data

ELISA - Human YKL-40 ELISA Kit (AB255719)

sELISA

Supplier Data

Sandwich ELISA - Human YKL-40 ELISA Kit (AB255719)

Interpolated concentrations of native YKL-40 in human urine, cell culture supernatant and cerebrospinal fluid (CSF) samples.

The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 100%, HepG2 cell culture supernatant 100%, U-87 MG cell culture supernatant 5%, and CSF 0.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 449.02 pg/mL in neat urine, 123.48 pg/mL in neat HepG2 cell culture supernatant, 18,524 pg/mL in neat U-87 MG cell culture supernatant and 145,710 pg/mL in neat CSF.

sELISA

Supplier Data

Sandwich ELISA - Human YKL-40 ELISA Kit (AB255719)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 42.74 ng/mL with a range of 16.87 – 78.32 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human YKL-40 ELISA Kit (AB255719)

Interpolated concentrations of native YKL-40 in human serum and plasma samples.

The concentrations of YKL-40 were measured in duplicates, interpolated from the YKL-40 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 40, plasma (citrate) 1 : 20, plasma (heparin) 1 : 20, and plasma (EDTA) 1 : 80. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean YKL-40 concentration was determined to be 37.59 ng/mL in neat serum, 16.17 ng/mL in neat plasma (citrate), 18.92 in neat plasma (heparin), and 67.92 ng/mL in neat plasma (EDTA).

sELISA

Unknown

Sandwich ELISA - Human YKL-40 ELISA Kit (AB255719)

Human YKL-40 standard curve comparison.

Standard Curve comparison between human YKL-40 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human YKL-40 ELISA Kit (AB255719)

Example of human YKL-40 standard curve in Sample Diluent NS.

The YKL-40 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cerebral Spinal Fluid, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

交差種

Human, Rhesus monkey

アッセイタイプ

Sandwich

Quantitative

検出感度

= 3.9 pg/mL

検出範囲

23.44 - 1500 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human YKL-40 ELISA Kit ab255719 is a rapid single-wash 90-min Sandwich ELISA to measure Human YKL-40 in cell culture supernatant, CSF, citrate plasma, EDTA plasma, heparin plasma, serum, urine. This SimpleStep sensitivity is 3.9 pg/mL.

How the assay works

Human YKL-40 SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human YKL-40 ELISA Kit ab255719 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-YKL-40/CHI3L1 antibody [EPR22243-81] - BSA and Azide free (Capture) ab259756
- Anti-YKL-40/CHI3L1 antibody [EPR22243-119] - BSA and Azide free (Detector) ab259756

YKL-40 (Chitinase-3-like protein 1) is a carbohydrate binding lectin with a preference for chitin. YKL-40 may play a role in tissue remodeling and cell response to changes in environment. It plays a the Th2 response of T-helper cells and IL-13-induced inflammation by regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and M2 macrophage differentiation. It regulated antibacterial response in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.4" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "100 - 107 %", "average": "= 103" }, { "sample": "Serum", "range": "101 - 122 %", "average": "= 110" }, { "sample": "EDTA Plasma", "range": "113 - 124 %", "average": "= 118" }, { "sample": "Cerebral Spinal Fluid", "range": "101 - 110 %", "average": "= 105" }, { "sample": "Urine", "range": "98 - 109 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "103 - 118 %", "average": "= 108" }, { "sample": "Citrate plasma", "range": "113 - 123 %", "average": "= 116" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.

Biological function summary

YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.

Pathways

YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-?) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.

YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.

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製品プロトコール

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ターゲットの情報

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.

See full target information CHI3L1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 24:1042 PubMed39179959

2024

Chitinase-3 like-protein-1, a prognostic biomarker in patients with hepatocellular carcinoma and concomitant myosteatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyu He,Zhihang Hu,Zuyuan Lin,Hao Chen,Chenghao Cao,Jinyan Chen,Xudong Yang,Huigang Li,Wei Shen,Xuyong Wei,Li Zhuang,Shusen Zheng,Xiao Xu,Di Lu

Journal of cellular and molecular medicine 27:1867-1879 PubMed37259630

2023

Anti-fibrotic effect of ciglitazone in HRV-induced airway remodelling cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna Wieczfinska,Rafal Pawliczak

The Libyan journal of medicine 18:2212480 PubMed37199347

2023

Increased YKL-40 levels are linked with disease severity of initially diagnosed Graves' disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ding Chen,Ci-You Huang,Zhuo-Ping Wang,Wen Wang

Aging 15:1199-1209 PubMed36880855

2023

Prognostic performance of serum YKL-40 for one-year clinical outcomes in acute ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Guomei Shi,Minghao Li,Yan E,Meng Wang,Pengyu Gong,Xiaorong Wang,Jingye Lu,Weixiang Wu,Shouru Xue,Junshan Zhou,Rujuan Zhou

Journal of inflammation research 16:311-319 PubMed36721527

2023

Serum YKL-40 Levels and White Matter Hyperintensities in Patients with Acute Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Guomei Shi,Dongdong Ke,Pengyu Gong,Peng Yu,Junshan Zhou,Meng Wang,Xiaohao Zhang,Xiaorong Wang,Minwang Guo,Mingyang Xu,Rujuan Zhou

Brain, behavior, & immunity - health 26:100555 PubMed36457825

2022

Conserved YKL-40 changes in mice and humans after postoperative delirium.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer David-Bercholz,Leah Acker,Ana I Caceres,Pau Yen Wu,Saanvi Goenka,Nathan O Franklin,Ramona M Rodriguiz,William C Wetsel,Michael Devinney,Mary Cooter Wright,Henrik Zetterberg,Ting Yang,Miles Berger,Niccolò Terrando

Journal of inflammation research 14:4361-4369 PubMed34511972

2021

The Relationship Between Serum YKL-40 Levels on Admission and Stroke-Associated Pneumonia in Patients with Acute Ischemic Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Guomei Shi,Wenxiu Chen,Pengyu Gong,Meng Wang,Junshan Zhou,Xiaorong Wang,Minwang Guo,Jingye Lu,Yan Li,Hongxuan Feng,Xuetao Fu,Rujuan Zhou,Shouru Xue

View all publications

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websiteProtocolBooklet

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Human YKL-40 ELISA Kit